Construct: ORF TRCN0000467301
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017763.1_s317c1
- Derived from:
- ccsbBroadEn_14482
- DNA Barcode:
- CACCGTCATCACTCAATCAATTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RGSL1 (353299)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467301
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 353299 | RGSL1 | regulator of G protein sign... | XM_017001197.2 | 91.9% | 91.7% | 1_138del;494G>T;562A>G |
| 2 | human | 353299 | RGSL1 | regulator of G protein sign... | XM_017001196.1 | 89.2% | 88.9% | 1_192del;548G>T;616A>G |
| 3 | human | 353299 | RGSL1 | regulator of G protein sign... | XM_024446727.1 | 89.2% | 88.9% | 1_192del;548G>T;616A>G |
| 4 | human | 353299 | RGSL1 | regulator of G protein sign... | XM_017001194.1 | 52.9% | 52.8% | 1_1422del;1778G>T;1846A>G |
| 5 | human | 353299 | RGSL1 | regulator of G protein sign... | NM_001137669.2 | 49.6% | 49.5% | 1_1623del;1979G>T;2047A>G |
| 6 | human | 353299 | RGSL1 | regulator of G protein sign... | XM_017001189.1 | 48.6% | 48.5% | 1_1689del;2045G>T;2113A>G |
| 7 | human | 353299 | RGSL1 | regulator of G protein sign... | XM_017001190.1 | 48.6% | 48.5% | 1_1689del;2045G>T;2113A>G |
| 8 | human | 353299 | RGSL1 | regulator of G protein sign... | XM_017001191.1 | 48.6% | 48.5% | 1_1689del;2045G>T;2113A>G |
| 9 | human | 353299 | RGSL1 | regulator of G protein sign... | XM_017001192.2 | 48.6% | 48.5% | 1_1689del;2045G>T;2113A>G |
| 10 | human | 353299 | RGSL1 | regulator of G protein sign... | XM_017001193.2 | 48.6% | 48.5% | 1_1689del;2045G>T;2113A>G |
| 11 | human | 353299 | RGSL1 | regulator of G protein sign... | NM_001366934.1 | 48% | 47.9% | 1_1728del;2084G>T;2152A>G |
| 12 | human | 353299 | RGSL1 | regulator of G protein sign... | XM_017001188.1 | 47.5% | 47.1% | (many diffs) |
| 13 | human | 353299 | RGSL1 | regulator of G protein sign... | XM_011509494.2 | 39.2% | 39.1% | (many diffs) |
| 14 | human | 353299 | RGSL1 | regulator of G protein sign... | XM_011509500.1 | 18.4% | 18.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1674
- ORF length:
- 1605
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggactatagg cagtggcgaa agatagctac tgaggacctg aagcaaggag 121 gctctctcca ggtagagctg acatctccag tgtttctaac agacataact aaaatgtcct 181 ttgaggagct ttgctacaag aacccaaaga tggccataca gaagatcagt gatgactaca 241 aaatatactg tgagaaagca cctaaaatag attttaaaat ggaaattatc aaggaaacaa 301 agacagtttc acgctcaaat aggaaaatgt ccttgctcaa aagaactctt gtaaggaagc 361 catcaatgag acccagaaac ttgacagaag tcctcttaaa tacacagcac ttggagttct 421 tcatggagtt cctcaaggaa cggaaggcta aaatcccatt gcaatttctc acagctgtac 481 agaagatcag tgtagagacc aatgaaaaga tttgcaagtc tctcatagaa aatgtaatca 541 agactttctt ccaaggccaa ctctctcctg aagaaatgct gcagtgtgat gcccctatta 601 tcaaagaaat cgcttccatg cgtcatgtca ccacaagcac actgttaacg ctccagggac 661 atgttatgaa atctatagaa gaaaagtggt ttaaagatta tcaagacctg ttcccacctc 721 accatcagga ggtggaagtg caaagtgaag tacaaatttc gtctaggaag ccctcaaaga 781 tagtgtcaac ttacctacag gaatcccaga agaaaggctg gatgagaatg atcagcttta 841 tcaggagttt ttgcaagtac cgcagattta tgttgaatcc tagtaagcgc caggaatttg 901 aagactatct tcaccaggaa atgcaaaata gcaaggaaaa tttcaccaca gcacacaaca 961 catcgggacg ttcagctcca ccctccacaa atgtccggag tgcagaccaa gagaatggag 1021 aaataaccct tgtaaagcgt cgtatatttg gccacaggat tatcactgtc aactttgcga 1081 tcaatgatct atatttcttt tctgaaatgg agaaatttaa tgatctggtc agttcagccc 1141 acatgctgca ggtcaaccgg gcatataatg agaatgatgt gatcctaatg cggtccaaaa 1201 tgaacattat ccaaaaactc ttcctgaatt ctgacatccc tccaaagctg agggtgaatg 1261 tcccTGAGTT CCAGAAGGAT GCCATCCTTG CTGCCATCAC AGAGGGCTAC CTAGATCGGA 1321 GCGTCTTCCA TGGGGCTATC ATGTCTGTCT TCCCCGTTGT TATGTACTTC TGGAAAAGGT 1381 TTTGTTTCTG GAAGGCAACC CGCTCTTACT TACAGTATAG GGGGAAGAAG TTCAAGGACA 1441 GAAAAAGCCC TCCTAAATCT ACGGACAAGT ATCCTTTCTC GAGTGGAGGA GACAATGCCA 1501 TCTTAAGGTT CACCTTGCTC AGAGGTATTG AGTGGTTGCA GCCTCAACGG GAAGCAATAA 1561 GTTCAGTTCA AAATTCTTCA TCAAGCAAAC TTACTCAGCC AAGACTCGTG GTATCTGCCA 1621 TGCAGCTGCA TCCCGTCCAG GGACAAAAAT TATCCTACAT CAAAAAAGAG AAGTTGCCAA 1681 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1741 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1801 TATCTTGTGG AAAGGACGAC ACCGTCATCA CTCAATCAAT TTCACGCGTT AAGTCgacaa 1861 tcaacctctg gattacaaaa tttgtgaaag att