Construct: ORF TRCN0000467313
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011749.1_s317c1
- Derived from:
- ccsbBroadEn_06364
- DNA Barcode:
- GGTTCGCGGGTTAAAGGTGATCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NRG1 (3084)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467313
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3084 | NRG1 | neuregulin 1 | NM_013959.3 | 99.8% | 99.6% | 397G>C |
| 2 | human | 3084 | NRG1 | neuregulin 1 | NM_001322207.2 | 59.5% | 59.2% | (many diffs) |
| 3 | human | 3084 | NRG1 | neuregulin 1 | NM_001322206.2 | 55.1% | 54.7% | (many diffs) |
| 4 | human | 3084 | NRG1 | neuregulin 1 | XM_005273487.3 | 42.5% | 40.7% | (many diffs) |
| 5 | human | 3084 | NRG1 | neuregulin 1 | XM_005273486.3 | 41.5% | 38.3% | (many diffs) |
| 6 | human | 3084 | NRG1 | neuregulin 1 | NM_001322205.2 | 41.2% | 40.8% | (many diffs) |
| 7 | mouse | 211323 | Nrg1 | neuregulin 1 | XM_017312637.1 | 48.5% | 47.7% | (many diffs) |
| 8 | mouse | 211323 | Nrg1 | neuregulin 1 | XM_017312636.1 | 37% | 35.4% | (many diffs) |
| 9 | mouse | 211323 | Nrg1 | neuregulin 1 | NM_178591.2 | 36.2% | 35.5% | (many diffs) |
| 10 | mouse | 211323 | Nrg1 | neuregulin 1 | XM_006509069.2 | 35% | 34.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 954
- ORF length:
- 888
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gatttattcc ccagacatgt ctgaggtcgc cgccgagagg tcctccagcc 121 cctccactca gctgagtgca gacccatctc ttgatgggct tccggcagca gaagacatgc 181 cagagcccca gactgaagat gggagaaccc ctggactcgt gggcctggcc gtgccctgct 241 gtgcgtgcct agaagctgag cgcctgagag gttgcctcaa ctcagagaaa atctgcattg 301 tccccatcct ggcttgcctg gtcagcctct gcctctgcat cgccggcctc aagtgggtat 361 ttgtggacaa gatctttgaa tatgactctc ctactcacct tgaccctggg gggttaggcc 421 aggaccctat tatttctctg gacgcaactg ctgcctcagc tctgtgggtg tcgtctgagg 481 catacacttc acctgtctct agggctcaat ctgaaagtga ggttcaagtt acagtgcaag 541 gtgacaaggc tgttgtctcc tttgaaccat cagcggcacc gacaccgaag aatcgtattt 601 ttgccttttc tttcttgccg tccactgcgc caTCCTTCCC TTCACCCACC CGGAACCCTG 661 AGGTGAGAAC GCCCAAGTCA GCAACTCAGC CACAAACAAC AGAAACTAAT CTCCAAACTG 721 CTCCTAAACT TTCTACATCT ACATCCACCA CTGGGACAAG CCATCTTGTA AAATGTGCGG 781 AGAAGGAGAA AACTTTCTGT GTGAATGGAG GGGAGTGCTT CATGGTGAAA GACCTTTCAA 841 ACCCCTCGAG ATACTTGTGC AAGTGCCCAA ATGAGTTTAC TGGTGATCGC TGCCAAAACT 901 ACGTAATGGC CAGCTTCTAC AGTACGTCCA CTCCCTTTCT GTCTCTGCCT GAATACCCAA 961 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1021 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1081 TATCTTGTGG AAAGGACGAG GTTCGCGGGT TAAAGGTGAT CCGACGCGTT AAGTCgacaa 1141 tcaacctctg gattacaaaa tttgtgaaag att