Construct: ORF TRCN0000467313

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011749.1_s317c1
Derived from:
ccsbBroadEn_06364
DNA Barcode:
GGTTCGCGGGTTAAAGGTGATCCG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NRG1 (3084)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000467313
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3084 NRG1 neuregulin 1 NM_013959.3 99.8% 99.6% 397G>C
2 human 3084 NRG1 neuregulin 1 NM_001322207.2 59.5% 59.2% (many diffs)
3 human 3084 NRG1 neuregulin 1 NM_001322206.2 55.1% 54.7% (many diffs)
4 human 3084 NRG1 neuregulin 1 XM_005273487.3 42.5% 40.7% (many diffs)
5 human 3084 NRG1 neuregulin 1 XM_005273486.3 41.5% 38.3% (many diffs)
6 human 3084 NRG1 neuregulin 1 NM_001322205.2 41.2% 40.8% (many diffs)
7 mouse 211323 Nrg1 neuregulin 1 XM_017312637.1 48.5% 47.7% (many diffs)
8 mouse 211323 Nrg1 neuregulin 1 XM_017312636.1 37% 35.4% (many diffs)
9 mouse 211323 Nrg1 neuregulin 1 NM_178591.2 36.2% 35.5% (many diffs)
10 mouse 211323 Nrg1 neuregulin 1 XM_006509069.2 35% 34.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
954
ORF length:
888
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga gatttattcc ccagacatgt ctgaggtcgc cgccgagagg tcctccagcc
121cctccactca gctgagtgca gacccatctc ttgatgggct tccggcagca gaagacatgc
181cagagcccca gactgaagat gggagaaccc ctggactcgt gggcctggcc gtgccctgct
241gtgcgtgcct agaagctgag cgcctgagag gttgcctcaa ctcagagaaa atctgcattg
301tccccatcct ggcttgcctg gtcagcctct gcctctgcat cgccggcctc aagtgggtat
361ttgtggacaa gatctttgaa tatgactctc ctactcacct tgaccctggg gggttaggcc
421aggaccctat tatttctctg gacgcaactg ctgcctcagc tctgtgggtg tcgtctgagg
481catacacttc acctgtctct agggctcaat ctgaaagtga ggttcaagtt acagtgcaag
541gtgacaaggc tgttgtctcc tttgaaccat cagcggcacc gacaccgaag aatcgtattt
601ttgccttttc tttcttgccg tccactgcgc caTCCTTCCC TTCACCCACC CGGAACCCTG
661AGGTGAGAAC GCCCAAGTCA GCAACTCAGC CACAAACAAC AGAAACTAAT CTCCAAACTG
721CTCCTAAACT TTCTACATCT ACATCCACCA CTGGGACAAG CCATCTTGTA AAATGTGCGG
781AGAAGGAGAA AACTTTCTGT GTGAATGGAG GGGAGTGCTT CATGGTGAAA GACCTTTCAA
841ACCCCTCGAG ATACTTGTGC AAGTGCCCAA ATGAGTTTAC TGGTGATCGC TGCCAAAACT
901ACGTAATGGC CAGCTTCTAC AGTACGTCCA CTCCCTTTCT GTCTCTGCCT GAATACCCAA
961CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG
1021ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA
1081TATCTTGTGG AAAGGACGAG GTTCGCGGGT TAAAGGTGAT CCGACGCGTT AAGTCgacaa
1141tcaacctctg gattacaaaa tttgtgaaag att

Download FASTA (ORF) (Full)