Transcript: Human XM_005273487.3

PREDICTED: Homo sapiens neuregulin 1 (NRG1), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
NRG1 (3084)
Length:
10379
CDS:
987..3065

Additional Resources:

NCBI RefSeq record:
XM_005273487.3
NBCI Gene record:
NRG1 (3084)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005273487.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000068234 GCCACAAACAACAGAAACTAA pLKO.1 1610 CDS 100% 5.625 3.938 N Nrg1 n/a
2 TRCN0000058307 TCATGGTGAAAGACCTTTCAA pLKO.1 1741 CDS 100% 0.563 0.394 N NRG1 n/a
3 TRCN0000204533 CCTCCCAAAGTGCTGGAATTA pLKO.1 10043 3UTR 100% 13.200 6.600 Y LRRC74B n/a
4 TRCN0000073773 CCTCTCAAGTAGCTGGGACTA pLKO.1 9893 3UTR 100% 4.050 2.025 Y TINAGL1 n/a
5 TRCN0000264189 CAAGTAGCTGGGACTACAGGA pLKO_005 9898 3UTR 100% 2.640 1.320 Y LINC01098 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005273487.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00736 pDONR223 100% 42.5% 40.9% None (many diffs) n/a
2 ccsbBroad304_00736 pLX_304 0% 42.5% 40.9% V5 (many diffs) n/a
3 ccsbBroadEn_06364 pDONR223 100% 42.5% 40.7% None (many diffs) n/a
4 ccsbBroad304_06364 pLX_304 0% 42.5% 40.7% V5 (many diffs) n/a
5 TRCN0000467313 GGTTCGCGGGTTAAAGGTGATCCG pLX_317 36.5% 42.5% 40.7% V5 (many diffs) n/a
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