Construct: ORF TRCN0000467322
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002263.2_s317c1
- Derived from:
- ccsbBroadEn_07382
- DNA Barcode:
- GTATTCAGCATCTCTCAATGAAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STK17B (9262)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467322
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9262 | STK17B | serine/threonine kinase 17b | NM_004226.4 | 99.7% | 100% | 126A>T;222A>G;741T>C |
| 2 | human | 9262 | STK17B | serine/threonine kinase 17b | XM_011512168.3 | 99.7% | 100% | 126A>T;222A>G;741T>C |
| 3 | human | 9262 | STK17B | serine/threonine kinase 17b | XM_011512169.1 | 99.7% | 100% | 126A>T;222A>G;741T>C |
| 4 | human | 9262 | STK17B | serine/threonine kinase 17b | XM_011512170.1 | 99.7% | 100% | 126A>T;222A>G;741T>C |
| 5 | human | 9262 | STK17B | serine/threonine kinase 17b | XM_011512171.2 | 65.5% | 65.5% | 0_1ins384;357T>C |
| 6 | mouse | 98267 | Stk17b | serine/threonine kinase 17b... | NM_133810.3 | 87.2% | 91.3% | (many diffs) |
| 7 | mouse | 98267 | Stk17b | serine/threonine kinase 17b... | XM_006496359.3 | 58.1% | 60.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1182
- ORF length:
- 1116
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc gaggaggaga tttgattgcc gaagtatttc aggcctacta actacaactc 121 ctcaaattcc aataaaaatg gaaaacttta ataatttcta tatacttaca tctaaagagc 181 tagggagagg taaatttgct gtggttagac aatgtatatc aaaatctact ggccaagaat 241 atgctgcaaa atttctaaaa aagagaagaa gaggacagga ttgtcgggca gaaattttac 301 acgagattgc tgtgcttgaa ttggcaaagt cttgtccccg tgttattaat cttcatgagg 361 tctatgaaaa tacaagtgaa atcattttga tattggaata tgctgcaggt ggagaaattt 421 tcagcctgtg tttacctgag ttggctgaaa tggtttctga aaatgatgtt atcagactca 481 ttaaacaaat acttgaagga gtttattatc tacatcagaa taacattgta caccttgatt 541 taaagccaca gaatatatta ctgagcagca tataccctct cggggacatt aaaatagtag 601 attttggaat gtctcgaaaa atagggcatg cgtgtgaact tcgggaaatc atgggaacac 661 cagaatattt agctccagaa atcctgaact atgatcccat taccacagca acagatatgt 721 ggaatattgg tataatagca tatatgttgt taactcacac atcaccattt gtgggagaag 781 ataatCAAGA AACATACCTC AATATCTCTC AAGTTAATGT AGATTATTCG GAAGAAACTT 841 TTTCATCAGT TTCACAGCTG GCCACAGACT TTATTCAGAG CCTTTTAGTA AAAAATCCAG 901 AGAAAAGACC AACAGCAGAG ATATGCCTTT CTCATTCTTG GCTACAGCAG TGGGACTTTG 961 AAAACTTGTT TCACCCTGAA GAAACTTCCA GTTCCTCTCA AACTCAGGAT CATTCTGTAA 1021 GGTCCTCTGA AGACAAGACT TCTAAATCCT CCTGTAATGG AACCTGTGGT GATAGAGAAG 1081 ACAAAGAGAA TATCCCAGAG GATAGCAGCA TGGTTTCCAA AAGATTTCGT TTCGATGACT 1141 CATTACCCAA TCCCCATGAA CTTGTTTCAG ATTTGCTCTG TTACCCAACT TTCTTGTACA 1201 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1261 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1321 AGGACGAGTA TTCAGCATCT CTCAATGAAG CACGCGTTAA GTCgacaatc aacctctgga 1381 ttacaaaatt tgtgaaagat t