Transcript: Mouse NM_133810.3

Mus musculus serine/threonine kinase 17b (apoptosis-inducing) (Stk17b), mRNA.

Source:
NCBI, updated 2017-05-27
Taxon:
Mus musculus (mouse)
Gene:
Stk17b (98267)
Length:
3298
CDS:
184..1302

Additional Resources:

NCBI RefSeq record:
NM_133810.3
NBCI Gene record:
Stk17b (98267)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_133810.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000024257 CAGAATAACATTGTTCACCTT pLKO.1 634 CDS 100% 2.640 3.696 N Stk17b n/a
2 TRCN0000024255 GCTGTGGTTAGACAATGTATA pLKO.1 316 CDS 100% 13.200 9.240 N Stk17b n/a
3 TRCN0000024258 TCCTCAACTATGATCCCATTA pLKO.1 800 CDS 100% 10.800 7.560 N Stk17b n/a
4 TRCN0000024254 CCCATGAACTTGTTCCAGATT pLKO.1 1271 CDS 100% 4.950 3.465 N Stk17b n/a
5 TRCN0000024256 GCTTGTTTCATCCTGAGGAAA pLKO.1 1082 CDS 100% 4.950 3.465 N Stk17b n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_133810.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000489402 TAGACTCGTCGCGTCGTCTGCCGT pLX_317 27.5% 87.3% 91.3% V5 (not translated due to prior stop codon) (many diffs) n/a
2 ccsbBroadEn_07382 pDONR223 100% 87.2% 91.3% None (many diffs) n/a
3 ccsbBroad304_07382 pLX_304 0% 87.2% 91.3% V5 (many diffs) n/a
4 TRCN0000467322 GTATTCAGCATCTCTCAATGAAGC pLX_317 35.8% 87.2% 91.3% V5 (many diffs) n/a
5 ccsbBroadEn_14936 pDONR223 0% 87.2% 91.3% None (many diffs) n/a
6 ccsbBroad304_14936 pLX_304 0% 87.2% 91.3% V5 (many diffs) n/a
7 TRCN0000471394 TCCACTCACTCCCTTAAACAAGTA pLX_317 38.7% 87.2% 91.3% V5 (many diffs) n/a
8 TRCN0000491895 AGCCCTGCGATTTCACCTTTTCGG pLX_317 22.2% 87.2% 91.1% V5 (many diffs) n/a
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