Construct: ORF TRCN0000467332
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001742.1_s317c1
- Derived from:
- ccsbBroadEn_09578
- DNA Barcode:
- CCTTACGGAAGTATAAACTGTTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PASD1 (139135)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467332
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 139135 | PASD1 | PAS domain containing repre... | NM_173493.3 | 99.9% | 100% | 33A>G |
| 2 | human | 139135 | PASD1 | PAS domain containing repre... | XM_011531102.2 | 99.8% | 99.8% | 33A>G;1434_1435insCAG |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2385
- ORF length:
- 2319
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gatgagaggg gaaaagagaa gagacaaggt caatccaaaa agctctcaaa 121 ggaaattaaa ctggattcca tcatttccta cctatgatta cttcaaccaa gtgacgctac 181 agttattaga tggctttatg attacactga gcacagatgg agtgatcatt tgtgtggctg 241 aaaacatctc ttctcttctt ggacatttac cagctgagat tgtgggcaaa aaattattaa 301 gccttctgcc tgatgaagag aaagatgaag tctaccaaaa gattattctc aaatttcctt 361 tactaaactc agaaacacat attgaatttt gctgtcattt aaaaagagga aatgtcgaac 421 atggtgatag ttctgcttac gaaaacgtga aatttattgt gaatgtaaga gatatttgta 481 atgagtttcc tgtggtcttt agtggcttgt tttccagcca cctctgtgct gactttgctg 541 catgtgttcc tcaggaggat cggctttatc ttgtgggaaa tgtttgcatt ctcaggactc 601 agctcctgca gcaactttac acttcaaagg cagtcagtga tgaagctgta cttacacaag 661 attcagatga ggaacctttt gtgggagagc tcagtagctc tcaaggtcaa agaggacaca 721 ctagcatgaa agccgtgtac gttgaacccg ctgctgctgc tgctgctgct gctatctcag 781 acgaccaaat tgatattgca gaggttgagc agtatggacc acaagaaaac gttcacatgt 841 ttgtagattc tgattcaact tattgctcca gtacagtttt cctggatact atgcctgaat 901 ctccagcctt atccttgcaa gactttcgag gtgagcctga ggtgaatcca ttgtacaggg 961 cagacccagt ggacctggag ttctcggtgg atcaggtgga ctcagtggac caggagggcc 1021 caatggacca gcaggaccca gagaacccag ttgccccgtt ggaccaggca ggcctgatgg 1081 atccagtgga tccagaggac tcagtggacc tgggggctgc tggcgcaagt gctcagccat 1141 tacagccatc atcaccagtt gcatatgaca tcattagcca ggaactggaa ctgatgaaga 1201 agttgaagga gcagctagaa gagaggactt ggttgctgca tgatgccatc caaaaccagc 1261 agaatgcatt ggaattgatg atggatcacc ttcagaagca gccaaacaca ttacgccacg 1321 ttgtcattcc tgatctccaa tcttcggagg cagtgcccaa gaaacaacag aaacaacacg 1381 ctgggcaagt gaagcggcct ctcccacatc ccaaggacgt caagtgtttc tgtggtttat 1441 ctttatccaa ctctctcaaa aacactgggg agcttcagga gccttgtgtt gccttcaacc 1501 agcagcaact ggtgcagcaa gaacaacacc tgaaggagca gcagcggcag ctgcgggagc 1561 agctgcaaca gctgagagag caaaggaagg tgcagaagca gaagaagatg caggagaaga 1621 agaagctgca ggagcagaaa atgcaggaga agaagaagct gcaggagcag aggcggcaaa 1681 agaagaagaa gctacaggag cggaagaagt ggcaggggca gatgctacag aaagagccag 1741 aggaggagca gcagaagcag cagctgcaag agcagccact gaagcataat gtcatcgtgg 1801 ggaatgagag ggtgcagata tgcctgcaaa acccacgtga cgtatctgtg cccctctgca 1861 atcaccctgt tagattttta caggcccaac ccattgttcc tgtccagaga gcagctgaac 1921 aacagccctc tggcttctat caagatgaaa actgtgggca acaggaagat gagagtcaaa 1981 gtttttatcc TGAGGCGTAT CAAGGGCCCC CCGTGAACCA GCTGCCATTG ATAGATACCT 2041 CAAACTCTGA GGCAATTTCT TCTTCCAGCA TTCCTCAGTT TCCCATAACT TCAGACTCAA 2101 CCATAAGCAC CCTGGAGACC CCACAGGATT ACATCCGGCT TTGGCAAGAG TTGTCTGATT 2161 CACTCGGTCC TGTTGTCCAA GTGAACACTT GGTCTTGCGA TGAGCAGGGC ACCCTGCACG 2221 GCCAACCCAC CTACCATCAG GTGCAAGTTT CTGAGGTAGG AGTCGAGGGA CCTCCTGATC 2281 CACAGGCTTT CCAAGGCCCT GCTGCATACC AGCCAGACCA GATGAGATCT GCGGAGCAGA 2341 CCAGATTGAT GCCTGCAGAG CAACGTGACT CAAATAAGCC GTGCTACCCA ACTTTCTTGT 2401 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 2461 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 2521 GAAAGGACGA CCTTACGGAA GTATAAACTG TTTTACGCGT TAAGTCgaca atcaacctct 2581 ggattacaaa atttgtgaaa gatt