Construct: ORF TRCN0000467349

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004147.1_s317c1
Derived from:
ccsbBroadEn_11743
DNA Barcode:
AAGCTACCCACTACACCAGGGGTA
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
PIK3R5 (23533)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000467349
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23533 PIK3R5 phosphoinositide-3-kinase r... NM_001251851.2 100% 3.9%
2 human 23533 PIK3R5 phosphoinositide-3-kinase r... NM_001251852.1 100% 3.9%
3 human 23533 PIK3R5 phosphoinositide-3-kinase r... NM_001251853.1 100% 3.9%
4 human 23533 PIK3R5 phosphoinositide-3-kinase r... NM_001251855.1 100% 3.9%
5 human 23533 PIK3R5 phosphoinositide-3-kinase r... NM_001142633.2 56.1% 1.4% 1_1158del
6 human 23533 PIK3R5 phosphoinositide-3-kinase r... NM_014308.3 56.1% 1.4% 1_1158del
7 human 23533 PIK3R5 phosphoinositide-3-kinase r... XM_005256580.3 56% 1.4% 1_1158del;1902_1903insCAG
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
58
ORF end:
265
ORF length:
207
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaatg
61ttggcatgga cagcggctac gtggaggaca gcgaggagag ctcctccgag tggccttgga
121ggcgtggcag ccaggaacgc cgaggccacc gcaggcctgg gcagaagttc atcaggatct
181ataaactctt caagagcacc agccagctgg tactgcggag ggactctcgg agcctggagg
241gcagctcgga cacggccctg cccctgaggc gggcagggag cctctgcagc cccctggacg
301aaccagtatc acccccttcc cgggcccagc gctcccgctc cctgccccag cccaaactcg
361gtacccagct gcccagctgg cttctggccc ctgcttcacg cccccagcgc cgccgcccct
421tcctgagtgg agatgaggat cccaaggctt ccacgctacg tgttgtggtc tttggctccg
481atcggatttc agggaaggtg gctcgggcgt acagcaacct tcggcggctg gagaacaatc
541gcccactcct cacacggttc ttcaaacttc agttcttcta cgtgcctgtg aagcgaagtc
601atgggaccag ccctggtgcc tgtccacccc ctcggagcca gacgccctca cccccgacag
661actcccctag gcacgccagc cctggagagc tgggcaccac cccatgggag gagagcacca
721atgacatctc ccactacctc ggcatgctgg acccctggta tgagcgcaat gtactgggcc
781tcatgcacct gccccctgaa gtcctgtgcc agcagtccct gaaggctgaa gcccaggccc
841tggagggctc cccaacccag ctgcccatcc tggctgacat gctactctac tactgccgct
901ttgccgccag accggtgctg ctgcaagtct atcagaccga gctgaccttc atcactgggg
961agaagacgac agagatcttc atccactcct tggagctggg tcactccgct gccacacgtg
1021ccatcaaggc gtcaggtcct ggcagcaagc ggctgggcat cgatggcgac cgggaggctg
1081ttcctctaac actacagatt atttacagca agggggccat cagtggacga agtcgctgga
1141gcaacctgga gaaggtctgt acctccgtga acctcaacaa ggccTGCCGG AAGCAGGAGG
1201AGCTGGATTC CAGCATGGAG GCCCTGACGC TAAACCTGAC AGAAGTGGTG AAAAGGCAGA
1261ACTCCAAATC CAAGAAGGGC TTTAACCAGA TTAGCACATC GCAGATCAAA GTGGACAAGG
1321TGCAGATCAT CGGCTCCAAC AGCTGCCCCT TTGCTGTGTG CCTGGACCAG GATGAGAGAA
1381AGATCCTGCA GAGTGTAGTC AGATGTGAGG TCTCACCGTG CTACAAGCCA GAGAAGAGCG
1441ACCTCTCCTC ACCACCCCAG ACGCCTCCTG ACCTGCCGGC CCAGGCCGCA CCTGATCTCT
1501GCTCCCTTCT CTGCCTGCCC ATCATGACTT TCAGTGGAGC TCTGCCCTAC CCAACTTTCT
1561TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA
1621CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT
1681GTGGAAAGGA CGAAAGCTAC CCACTACACC AGGGGTAACG CGTTAAGTCg acaatcaacc
1741tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)