Construct: ORF TRCN0000467380
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007591.1_s317c1
- Derived from:
- ccsbBroadEn_08829
- DNA Barcode:
- AGCCAGCAACAATTAACCAACAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MCCC2 (64087)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467380
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64087 | MCCC2 | methylcrotonoyl-CoA carboxy... | NM_022132.5 | 99.9% | 100% | 1368A>G |
| 2 | human | 64087 | MCCC2 | methylcrotonoyl-CoA carboxy... | NM_001363147.1 | 93.1% | 93.2% | 623_624ins114;1254A>G |
| 3 | human | 64087 | MCCC2 | methylcrotonoyl-CoA carboxy... | XM_011543529.2 | 62.6% | 60% | (many diffs) |
| 4 | human | 64087 | MCCC2 | methylcrotonoyl-CoA carboxy... | XM_017009688.1 | 62.5% | 59.8% | (many diffs) |
| 5 | human | 64087 | MCCC2 | methylcrotonoyl-CoA carboxy... | XR_001742172.1 | 46.5% | (many diffs) | |
| 6 | mouse | 78038 | Mccc2 | methylcrotonoyl-Coenzyme A ... | NM_030026.2 | 84.1% | 88.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1755
- ORF length:
- 1689
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg ggccgtcctg aggttagccc tgcggccgtg tgcccgcgcc tctcccgccg 121 ggccgcgcgc ctatcacggg gactcggtgg cctcgctggg cacccagccg gacttgggct 181 ctgccctcta ccaggagaac tacaagcaga tgaaagcact agtaaatcag ctccatgaac 241 gagtggagca tataaaacta ggaggtggtg agaaagcccg agcacttcac atatcaagag 301 gaaaactatt gcccagagaa agaattgaca atctcataga cccagggtct ccatttctgg 361 aattatccca gtttgcaggt taccagttat atgacaatga ggaggtgcca ggaggtggca 421 ttattacagg cattggaaga gtatcaggag tagaatgcat gattattgcc aatgatgcca 481 ccgtcaaagg aggtgcctac tacccagtga ctgtgaaaaa acaattacgg gcccaagaaa 541 ttgccatgca aaacaggctc ccctgcatct acttagttga ttcgggagga gcatacttac 601 ctcgacaagc agatgtgttt ccagatcgag accactttgg ccgtacattc tataatcagg 661 caattatgtc ttctaaaaat attgcacaga tcgcagtggt catgggctcc tgcaccgcag 721 gaggagccta tgtgcctgcc atggctgatg aaaacatcat tgtacgcaag cagggtacca 781 ttttcttggc aggacccccc ttggttaaag cggcaactgg ggaagaagta tctgctgagg 841 atcttggagg tgctgatctt cattgcagaa agtctggagt aagtgaccac tgggctttgg 901 atgatcatca tgcccttcac ttaactagga aggttgtgag gaatctaaat tatcagaaga 961 aattggatgt caccattgaa ccttctgaag agcctttatt tcctgctgat gaattgtatg 1021 gaatagttgg tgctaacctt aagaggagct ttgatgtccg agaggtcatt gctagaatcg 1081 tggatggaag cagattcact gagttcaaag ccttttatgg agacacatta gttacaggat 1141 ttgctcgaat atttgggtac ccagtaggta tcgttggaaa caacggagtt ctcttttctg 1201 aatctgcaaa aaagggtact cactttgtcc agttatgctg ccaaagaaat attcctctgc 1261 tgttccttca aaacattact ggatttatgg ttggtagaga gtatgaagct gaaggaattg 1321 ccaaggatgg tgccaagatg gtggccgctg tggcctgtgc ccaagtgcct aagataaccc 1381 tcatcattgg gggctcctat GGAGCCGGAA ACTATGGGAT GTGTGGCAGA GCGTATAGCC 1441 CAAGATTTCT CTACATTTGG CCAAATGCTC GTATCTCAGT GATGGGAGGA GAGCAGGCAG 1501 CCAATGTGTT GGCCACGATA ACAAAGGACC AAAGAGCCCG GGAAGGAAAG CAGTTCTCCA 1561 GTGCTGATGA AGCGGCTTTA AAAGAGCCCA TCATTAAGAA GTTTGAAGAG GAAGGAAACC 1621 CTTACTATTC CAGCGCAAGG GTATGGGATG ATGGGATCAT TGATCCAGCA GACACCAGAC 1681 TGGTCTTGGG TCTCAGTTTT AGTGCAGCCC TCAACGCACC AATAGAGAAG ACTGACTTCG 1741 GTATCTTCAG GATGTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1801 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1861 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA AGCCAGCAAC AATTAACCAA 1921 CAATACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt