Construct: ORF TRCN0000467393
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001875.1_s317c1
- Derived from:
- ccsbBroadEn_00793
- DNA Barcode:
- CTCCCACTTTAGCCATCCTACATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HSD17B4 (3295)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467393
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3295 | HSD17B4 | hydroxysteroid 17-beta dehy... | NM_000414.4 | 100% | 100% | |
| 2 | human | 3295 | HSD17B4 | hydroxysteroid 17-beta dehy... | NM_001199292.2 | 97.5% | 97.5% | 57_58ins54 |
| 3 | human | 3295 | HSD17B4 | hydroxysteroid 17-beta dehy... | NM_001292027.2 | 95.6% | 94.9% | (many diffs) |
| 4 | human | 3295 | HSD17B4 | hydroxysteroid 17-beta dehy... | NM_001199291.3 | 95.2% | 91.4% | (many diffs) |
| 5 | human | 3295 | HSD17B4 | hydroxysteroid 17-beta dehy... | NM_001292028.2 | 80.9% | 80.9% | 0_1ins411;14_15insGTATGGAAG |
| 6 | mouse | 15488 | Hsd17b4 | hydroxysteroid (17-beta) de... | NM_008292.4 | 84.8% | 86.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2274
- ORF length:
- 2208
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg ctcaccgctg aggttcgacg ggcgggtggt actggtcacc ggcgcggggg 121 caggattggg ccgagcctat gccctggctt ttgcagaaag aggagcgtta gttgttgtga 181 atgatttggg aggggacttc aaaggagttg gtaaaggctc cttagctgct gataaggttg 241 ttgaagaaat aagaaggaga ggtggaaaag cagtggccaa ctatgattca gtggaagaag 301 gagagaaggt tgtgaagaca gccctggatg cttttggaag aatagatgtt gtggtcaaca 361 atgctggaat tctgagggat cgttcctttg ctaggataag tgatgaagac tgggatataa 421 tccacagagt tcatttgcgg ggttcattcc aagtgacacg ggcagcatgg gaacacatga 481 agaaacagaa gtatggaagg attattatga cttcatcagc ttcaggaata tatggcaact 541 ttggccaggc caattatagt gctgcaaagt tgggtcttct gggccttgca aattctcttg 601 caattgaagg caggaaaagc aacattcatt gtaacaccat tgctcctaat gcgggatcac 661 ggatgactca gacagttatg cctgaagatc ttgtggaagc cctgaagcca gagtatgtgg 721 cacctcttgt cctttggctt tgtcacgaga gttgtgagga gaatggtggc ttgtttgagg 781 ttggagcagg atggattgga aaattacgct gggagcggac tcttggagct attgtaagac 841 aaaagaatca cccaatgact cctgaggcag tcaaggctaa ctggaagaag atctgtgact 901 ttgagaatgc cagcaagcct cagagtatcc aagaatcaac tggcagtata attgaagttc 961 tgagtaaaat agattcagaa ggaggagttt cagcaaatca tactagtcgt gcaacgtcta 1021 cagcaacatc aggatttgct ggagctattg gccagaaact ccctccattt tcttatgctt 1081 atacggaact ggaagctatt atgtatgccc ttggagtggg agcgtcaatc aaggatccaa 1141 aagatttgaa atttatttat gaaggaagtt ctgatttctc ctgtttgccc accttcggag 1201 ttatcatagg tcagaaatct atgatgggtg gaggattagc agaaattcct ggactttcaa 1261 tcaactttgc aaaggttctt catggagagc agtacttaga gttatataaa ccacttccca 1321 gagcaggaaa attaaaatgt gaagcagttg ttgctgatgt cctagataaa ggatccggtg 1381 tagtgattat tatggatgtc tattcttatt ctgagaagga acttatatgc cacaatcagt 1441 tctctctctt tcttgttggc tctggaggct ttggtggaaa acggacatca gacaaagtca 1501 aggtagctgt agccatacct aatagacctc ctgatgctgt acttacagat accacctctc 1561 ttaatcaggc tgctttgtac cgcctcagtg gagactggaa tcccttacac attgatccta 1621 actttgctag tctagcaggt tttgacaagc ccatattaca tggattatgt acatttggat 1681 tttctgccag gcgtgtgtta cagcagtttg cagataatga tgtgtcaaga ttcaaggcaa 1741 ttaaggctcg ttttgcaaaa ccagtatatc caggacaaac tctacaaact gagatgtgga 1801 aggaaggaaa cagaattcat tttcaaacca aggtccaaga aactGGAGAC ATTGTCATTT 1861 CAAATGCATA TGTGGATCTT GCACCAACAT CTGGTACTTC AGCTAAGACA CCCTCTGAGG 1921 GCGGGAAGCT TCAGAGTACC TTTGTATTTG AGGAAATAGG ACGCCGCCTA AAGGATATTG 1981 GGCCTGAGGT GGTGAAGAAA GTAAATGCTG TATTTGAGTG GCATATAACC AAAGGCGGAA 2041 ATATTGGGGC TAAGTGGACT ATTGACCTGA AAAGTGGTTC TGGAAAAGTG TACCAAGGCC 2101 CTGCAAAAGG TGCTGCTGAT ACAACAATCA TACTTTCAGA TGAAGATTTC ATGGAGGTGG 2161 TCCTGGGCAA GCTTGACCCT CAGAAGGCAT TCTTTAGTGG CAGGCTGAAG GCCAGAGGGA 2221 ACATCATGCT GAGCCAGAAA CTTCAGATGA TTCTTAAAGA CTACGCCAAG CTCTGCCCAA 2281 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 2341 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 2401 TATCTTGTGG AAAGGACGAC TCCCACTTTA GCCATCCTAC ATAACGCGTT AAGTCgacaa 2461 tcaacctctg gattacaaaa tttgtgaaag att