Construct: ORF TRCN0000467398

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002737.1_s317c1
Derived from:
ccsbBroadEn_07309
DNA Barcode:
TCAGGTGCATTTAGCAAGACACTC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DPM1 (8813)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000467398
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 8813 DPM1 dolichyl-phosphate mannosyl... NM_003859.2 99.8% 99.6% 779C>T
2 human 8813 DPM1 dolichyl-phosphate mannosyl... NM_001317036.1 91% 90.7% 490_491ins69;710C>T
3 human 8813 DPM1 dolichyl-phosphate mannosyl... NM_001317035.1 90.4% 90.2% 492_572del;860C>T
4 human 8813 DPM1 dolichyl-phosphate mannosyl... NM_001317034.1 88% 87.7% 492_596del;884C>T
5 human 8813 DPM1 dolichyl-phosphate mannosyl... XR_002958550.1 69.3% (many diffs)
6 human 8813 DPM1 dolichyl-phosphate mannosyl... NR_133648.1 69.1% (many diffs)
7 human 8813 DPM1 dolichyl-phosphate mannosyl... XR_002958551.1 62.2% (many diffs)
8 mouse 13480 Dpm1 dolichol-phosphate (beta-D)... NM_010072.4 87.2% 90.7% (many diffs)
9 mouse 13480 Dpm1 dolichol-phosphate (beta-D)... NM_001310084.1 79.5% 82.3% (many diffs)
10 mouse 105980075 Gm20716 predicted gene 20716 NR_132310.1 12.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
846
ORF length:
780
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc ctccttggaa gtcagtcgta gtcctcgcag gtctcggcgg gagctggaag
121tgcgcagtcc acgacagaac aaatattcgg tgcttttacc tacctacaac gagcgcgaga
181acctgccgct catcgtgtgg ctgctggtga aaagcttctc cgagagtgga atcaactatg
241aaattataat catagatgat ggaagcccag atggaacaag ggatgttgct gaacagttgg
301agaagatcta tgggtcagac agaattcttc taagaccacg agagaaaaag ttgggactag
361gaactgcata tattcatggA ATGAAACATG CCACAGGAAA CTACATCATT ATTATGGATG
421CTGATCTCTC ACACCATCCA AAATTTATTC CTGAATTTAT TAGGAAGCAA AAGGAGGGTA
481ATTTTGATAT TGTCTCTGGA ACTCGCTACA AAGGAAATGG AGGTGTATAT GGCTGGGATT
541TGAAAAGAAA AATAATCAGC CGTGGGGCCA ATTTTTTAAC TCAGATCTTG CTGAGACCAG
601GAGCATCTGA TTTAACAGGA AGTTTCAGAT TATACCGAAA AGAAGTTCTA GAGAAATTAA
661TAGAAAAATG TGTTTCTAAA GGCTACGTCT TCCAGATGGA GATGATTGTT CGGGCAAGAC
721AGTTGAATTA TACTATTGGC GAGGTTCCAA TATCATTTGT GGATCGTGTT TATGGTGAAT
781CCAAGTTGGG AGGAAATGAA ATAGTATCTT TCTTGAAAGG ATTATTGACT CTTTTTGCTA
841CTATATACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC
901TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT
961CTTGGCTTTA TATATCTTGT GGAAAGGACG ATCAGGTGCA TTTAGCAAGA CACTCACGCG
1021TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt

Download FASTA (ORF) (Full)