Construct: ORF TRCN0000467404
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015770.1_s317c1
- Derived from:
- ccsbBroadEn_02641
- DNA Barcode:
- TGCCATGCGGCTGGGATGGCTATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STRAP (11171)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467404
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11171 | STRAP | serine/threonine kinase rec... | NM_007178.4 | 100% | 100% | |
| 2 | human | 11171 | STRAP | serine/threonine kinase rec... | XM_005253294.2 | 73.1% | 73.1% | 0_1ins282 |
| 3 | mouse | 20901 | Strap | serine/threonine kinase rec... | NM_011499.3 | 90.6% | 97.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1116
- ORF length:
- 1050
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc aatgagacag acgccgctca cctgctctgg ccacacgcga cccgtggttg 121 atttggcctt cagtggcatc acgccttatg ggtatttctt aatcagcgct tgcaaagatg 181 gtaaacctat gctacgccag ggagatacag gagactggat tggaacattt ttgggtcata 241 aaggtgctgt ttggggtgca acactgaata aggatgccac caaagcagct acagcagctg 301 cagatttcac agccaaagtg tgggatgctg tctcaggaga tgaattgatg accctggctc 361 ataaacacat tgtcaagact gtggatttca cgcaggatag taattatttg ttaaccgggg 421 gacaggataa actgttacgc atatatgact tgaacaaacc tgaagcagaa cctaaggaaa 481 ttagtggtca tacttctggt ataaaaaaag ctctgtggtg cagtgaggat aaacagattc 541 tttctgctga tgacaaaact gttcgacttt gggatcatgc tactatgaca gaagtgaaat 601 ctctaaattt taatatgtct gttagtagta tggaatatat tccTGAGGGA GAGATTTTGG 661 TTATAACTTA TGGACGATCT ATTGCTTTTC ATAGTGCAGT AAGTTTGGAC CCAATTAAAT 721 CCTTTGAAGC TCCTGCAACC ATCAATTCTG CATCTCTTCA TCCTGAGAAA GAATTTCTTG 781 TTGCAGGCGG TGAAGATTTT AAACTTTATA AGTATGATTA TAATAGTGGA GAAGAATTAG 841 AATCCTACAA GGGACACTTT GGTCCTATTC ACTGTGTGAG ATTTAGTCCT GATGGAGAAC 901 TCTATGCCAG TGGTTCAGAA GATGGAACAT TGAGACTATG GCAAACTGTG GTAGGAAAAA 961 CGTATGGCCT TTGGAAATGT GTGCTTCCTG AAGAAGATAG TGGTGAGCTG GCAAAGCCAA 1021 AGATTGGTTT TCCAGAGACA ACAGAAGAGG AGCTAGAAGA AATTGCTTCA GAGAATTCAG 1081 ATTGCATCTT TCCTTCAGCT CCTGATGTTA AGGCCTGCCC AACTTTCTTG TACAAAGTGG 1141 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1201 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1261 ATGCCATGCG GCTGGGATGG CTATCACGCG TTAAGTCgac aatcaacctc tggattacaa 1321 aatttgtgaa agatt