Construct: ORF TRCN0000467412

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011802.1_s317c1
Derived from:
ccsbBroadEn_06140
DNA Barcode:
CATAATCATGTCTCCCTTCAGCCG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
EEF1D (1936)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000467412
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1936 EEF1D eukaryotic translation elon... NM_001130053.4 99.8% 99.8% 330C>T;567C>G;1008G>T
2 human 1936 EEF1D eukaryotic translation elon... NM_032378.6 99.8% 99.8% 330C>T;567C>G;1008G>T
3 human 1936 EEF1D eukaryotic translation elon... XM_005250820.4 99.8% 99.8% 330C>T;567C>G;1008G>T
4 human 1936 EEF1D eukaryotic translation elon... XM_005250823.1 99.8% 99.8% 330C>T;567C>G;1008G>T
5 human 1936 EEF1D eukaryotic translation elon... XM_006716522.1 99.8% 99.8% 330C>T;567C>G;1008G>T
6 human 1936 EEF1D eukaryotic translation elon... XM_006716523.2 99.8% 99.8% 330C>T;567C>G;1008G>T
7 human 1936 EEF1D eukaryotic translation elon... XM_006716524.1 99.8% 99.8% 330C>T;567C>G;1008G>T
8 human 1936 EEF1D eukaryotic translation elon... XM_011516905.2 99.8% 99.8% 330C>T;567C>G;1008G>T
9 human 1936 EEF1D eukaryotic translation elon... XM_011516906.3 99.8% 99.8% 330C>T;567C>G;1008G>T
10 human 1936 EEF1D eukaryotic translation elon... XM_017013170.2 99.8% 99.8% 330C>T;567C>G;1008G>T
11 human 1936 EEF1D eukaryotic translation elon... XM_006716519.4 99.6% 99.5% (many diffs)
12 human 1936 EEF1D eukaryotic translation elon... XM_017013171.1 99.6% 99.5% (many diffs)
13 human 1936 EEF1D eukaryotic translation elon... XM_024447088.1 99.6% 99.5% (many diffs)
14 human 1936 EEF1D eukaryotic translation elon... XM_006716520.4 96.1% 96.1% (many diffs)
15 human 1936 EEF1D eukaryotic translation elon... XM_017013172.1 96.1% 96.1% (many diffs)
16 human 1936 EEF1D eukaryotic translation elon... XM_024447089.1 96.1% 96.1% (many diffs)
17 human 1936 EEF1D eukaryotic translation elon... NM_001130055.3 43.4% 43.4% 0_1ins1098
18 human 1936 EEF1D eukaryotic translation elon... NM_001130057.3 43.4% 43.4% 0_1ins1098
19 human 1936 EEF1D eukaryotic translation elon... NM_001289950.3 43.4% 43.4% 0_1ins1098
20 human 1936 EEF1D eukaryotic translation elon... NM_001960.6 43.4% 43.4% 0_1ins1098
21 human 1936 EEF1D eukaryotic translation elon... XM_006716525.1 43.4% 43.4% 0_1ins1098
22 human 1936 EEF1D eukaryotic translation elon... NM_001195203.3 40.4% 40.4% 0_1ins1098;230_231ins57
23 human 1936 EEF1D eukaryotic translation elon... NM_001130056.4 39.7% 39.7% 0_1ins1098;117_118ins72
24 human 1936 EEF1D eukaryotic translation elon... NM_001317743.3 39.7% 39.7% 0_1ins1098;117_118ins72
25 human 1936 EEF1D eukaryotic translation elon... NM_001330646.2 39.7% 39.7% 0_1ins1098;117_118ins72
26 mouse 66656 Eef1d eukaryotic translation elon... NM_029663.2 83.1% 83.9% (many diffs)
27 mouse 66656 Eef1d eukaryotic translation elon... XM_006521264.3 83% 83.6% (many diffs)
28 mouse 66656 Eef1d eukaryotic translation elon... XM_006521261.3 80.8% 81.5% (many diffs)
29 mouse 66656 Eef1d eukaryotic translation elon... XM_006521266.3 80.8% 81.5% (many diffs)
30 mouse 66656 Eef1d eukaryotic translation elon... XM_006521267.3 80.8% 81.5% (many diffs)
31 mouse 66656 Eef1d eukaryotic translation elon... XM_006521271.1 42.2% 41.7% (many diffs)
32 mouse 66656 Eef1d eukaryotic translation elon... XM_006521272.2 42% 41.3% (many diffs)
33 mouse 66656 Eef1d eukaryotic translation elon... XM_017316725.1 41% 40.5% (many diffs)
34 mouse 66656 Eef1d eukaryotic translation elon... NM_001285429.1 37.6% 40.1% (many diffs)
35 mouse 66656 Eef1d eukaryotic translation elon... NM_001285430.1 37.6% 40.1% (many diffs)
36 mouse 66656 Eef1d eukaryotic translation elon... NM_023240.3 37.6% 40.1% (many diffs)
37 mouse 66656 Eef1d eukaryotic translation elon... XM_006521268.3 36.5% 39% (many diffs)
38 mouse 66656 Eef1d eukaryotic translation elon... XM_006521273.2 36.5% 39% (many diffs)
39 mouse 66656 Eef1d eukaryotic translation elon... NM_001285434.1 35.6% 37% (many diffs)
40 mouse 66656 Eef1d eukaryotic translation elon... NM_001285431.1 34.1% 36.4% (many diffs)
41 mouse 66656 Eef1d eukaryotic translation elon... NM_001285432.1 34.1% 36.4% (many diffs)
42 mouse 66656 Eef1d eukaryotic translation elon... NM_001285433.1 34.1% 36.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2007
ORF length:
1941
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgag gagcgggaag gcctcctgca ccctggagac cgtgtgggaa gacaagcaca
121agtatgagga ggccgagcgg cgcttctacg aacacgaggc cacacaggcg gccgcctccg
181cccagcagct gccagccgag gggccagcca tgaatgggcc cggccaggac gaccctgagg
241acgctgatga ggcggaagcc cctgacggcg gcagcaggcg tgatcccagg aagagccagg
301acagcaggaa gcccctgcag aaaaagagga agcgctcccc caagagcggg ctcggccccg
361cggacctggc cctcctgggc ctctcggccg aacgtgtgtg gctggacaag tcacttttcg
421accaggcaga gagctcctac cgccagaagc tggcagatgt ggctgcccag gcagcctggc
481ctcctgcctt ggccccttgg ggtctctgca cccatggaaa ccaggtggcc tgccaccacg
541tgacctgggg gatctgggtc aacaagtcct ccttcgacca ggctgagcgg gccttcgtgg
601agtggtctca ggccctgttg ctggcccccg agggcagccg caggcagggg actcccaaca
661caggccagca ggtggccgtc cccgacctgg cccaccagcc cagcccaccg gtcaatggcc
721agcccccgct gggcagcctg caggcactgg ttcgggaggt gtggctggag aagccccggt
781atgatgcagc cgagaggggc ttctacgagg ccctgtttga cggccatccc ccagggaagg
841tgcgcctgca agagcgagcc ggcctggccg agggtgcccg gcggggccgc agagaccggc
901ggggccgcaa catcttaggg aacaagcggg ccgggctgcg acgggccgat ggggaggccc
961cctctgcctt gccctactgt tacttcctgc agaaggatgc agaggccccc tggctcagca
1021agcctgccta cgacagcgcc gagtgccgcc accacgctgc cgaggccctg cgtgtggcct
1081ggtgcctcga agctgcctcc ctgtctcacc gacccggtcc tcggtctggc ctgtccgtgt
1141ccagcctgag acccaacaga aaaatggcta caaacttcct agcacatgag aagatctggt
1201tcgacaagtt caaatatgac gacgcagaaa ggagattcta cgagcagatg aacgggcctg
1261tggcaggtgc ctcccgccag gagaacggcg ccagcgtgat cctccgtgac attgcgagag
1321ccagagagaa catccagaaa tccctggctg gaagctcagg ccccggggcc tccagcggca
1381ccagcggaga ccacggtgag ctcgtcgtcc ggattgccag tctggaagtg gagaaccaga
1441gtctgcgtgg cgtggtacag gagctgcagc aggccatctc caagctggag gcccggctga
1501acgtgctgga gaagagctcg cctggccacc gggccacggc cccacagacc cagcacgtat
1561ctcccatgcg ccaagtggag cccccagcca agaagccagc cacaccagca gaggatgacg
1621aggatgatga cattgacctg tttggcagtg acaatgagga ggaggacaag gaggcggcac
1681agctgcggga ggagcggcta cggcagtacg cggagaagaa ggccaagaag cctgcactgg
1741tggccaagtc ctccatcctg ctggatgtca agccttggga tgatGAGACG GACATGGCCC
1801AGCTGGAGGC CTGTGTGCGC TCTATCCAGC TGGACGGGCT GGTCTGGGGG GCTTCCAAGC
1861TGGTGCCCGT GGGCTACGGT ATCCGGAAGC TACAGATTCA GTGTGTGGTG GAGGACGACA
1921AGGTGGGGAC AGACTTGCTG GAGGAGGAGA TCACCAAGTT TGAGGAGCAC GTGCAGAGTG
1981TCGATATCGC AGCTTTCAAC AAGATCTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG
2041GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGCCCGT
2101AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACATAATCA
2161TGTCTCCCTT CAGCCGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga
2221aagatt

Download FASTA (ORF) (Full)