Construct: ORF TRCN0000467462
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010233.1_s317c1
- Derived from:
- ccsbBroadEn_07952
- DNA Barcode:
- TTGTGAGCGCACTTAAGCTAAGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PARM1 (25849)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467462
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25849 | PARM1 | prostate androgen-regulated... | NM_015393.4 | 99.7% | 99.6% | 39T>C;367G>A |
| 2 | human | 25849 | PARM1 | prostate androgen-regulated... | XM_011531833.1 | 89.6% | 89.5% | 39T>C;43_147del;472G>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 996
- ORF length:
- 930
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt ctacaagact ctcttcgctc tttgcatctt aaccgcagga tggagggtac 121 agagtctgcc tacatcagct cctttgtctg tttctcttcc gacaaacatt gtaccaccga 181 ccaccatctg gactagctct ccacaaaaca ctgatgcaga cactgcctcc ccatccaacg 241 gcactcacaa caactcggtg ctcccagtta cagcatcagc cccaacatct ctgcttccta 301 agaacatttc catagagtcc agagaagagg agatcaccag cccaggttcg aattgggaag 361 gcacaaacac agacccctca ccttctgggt tctcgtcaac aagcggtgga gtccacttaa 421 caaccacgtt gaaggaacac agctcgggca ctcctgaagc aggcgtggca gctacactgt 481 cgcagtccgc tgctgagcct cccacactca tctcccctca agctccagcc tcatcaccct 541 catccctatc aacctcacca cctgaggtct tttctgccTC CGTTACTACC AACCATAGCT 601 CCACTGTGAC CAGCACCCAA CCCACTGGAG CTCCAACTGC ACCAGAGTCC CCGACAGAGG 661 AGTCCAGCTC TGACCACACA CCCACTTCAC ATGCCACAGC TGAGCCAGTA CCCCAGGAGA 721 AAACACCCCC AACAACTGTG TCAGGCAAAG TGATGTGTGA GCTCATAGAC ATGGAGACCA 781 CCACCACCTT TCCCAGGGTG ATCATGCAGG AAGTAGAACA TGCATTAAGT TCAGGCAGCA 841 TCGCCGCCAT TACCGTGACA GTCATTGCCG TGGTGCTGCT GGTGTTTGGA GTTGCAGCCT 901 ACCTAAAAAT CAGGCATTCC TCCTATGGAA GACTTTTGGA CGACCATGAC TACGGGTCCT 961 GGGGAAACTA CAACAACCCT CTGTACGATG ACTCCTACCC AACTTTCTTG TACAAAGTGG 1021 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1081 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1141 ATTGTGAGCG CACTTAAGCT AAGCCACGCG TTAAGTCgac aatcaacctc tggattacaa 1201 aatttgtgaa agatt