Construct: ORF TRCN0000467471
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012378.1_s317c1
- Derived from:
- ccsbBroadEn_11015
- DNA Barcode:
- TCTTACTCACATTGAGATACATTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRKN (5071)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467471
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5071 | PRKN | parkin RBR E3 ubiquitin pro... | NM_004562.3 | 75.4% | 75% | (many diffs) |
| 2 | human | 5071 | PRKN | parkin RBR E3 ubiquitin pro... | XM_011535863.1 | 75.2% | 74.7% | (many diffs) |
| 3 | human | 5071 | PRKN | parkin RBR E3 ubiquitin pro... | XM_017010908.1 | 69.8% | 69.1% | (many diffs) |
| 4 | human | 5071 | PRKN | parkin RBR E3 ubiquitin pro... | NM_013987.3 | 69.6% | 69.2% | (many diffs) |
| 5 | human | 5071 | PRKN | parkin RBR E3 ubiquitin pro... | XM_024446449.1 | 59.1% | 58.6% | (many diffs) |
| 6 | human | 5071 | PRKN | parkin RBR E3 ubiquitin pro... | XM_017010909.2 | 58.9% | 58.4% | (many diffs) |
| 7 | human | 5071 | PRKN | parkin RBR E3 ubiquitin pro... | NM_013988.3 | 44.8% | 44.2% | (many diffs) |
| 8 | human | 5071 | PRKN | parkin RBR E3 ubiquitin pro... | XR_001743443.2 | 25.2% | (many diffs) | |
| 9 | mouse | 50873 | Park2 | Parkinson disease (autosoma... | XM_006523339.2 | 31.2% | 32.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1227
- ORF length:
- 1161
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat agtgtttgtc aggttcaact ccagccatgg tttcccagtg gaggtcgatt 121 ctgacaccag catcttccag ctcaaggagg tggttgctaa gcgacagggg gttccggctg 181 accagttgcg tgtgattttc gcagggaagg agctgaggaa tgactggact gtgcagaatt 241 gtgacctgga tcagcagagc attgttcaca ttgtgcagag accgtggaga aaaggtcaag 301 aaatgaatgc aactggaggc gacgacccca gaaacgcggc gggaggctgt gagcgggagc 361 cccagagctt gactcgggtg gacctcagca gctcagtcct cccaggagac tctgtggggc 421 tggctgtcat tctgcacact gacagcagga aggactcacc accagctgga agtccagcag 481 gtagatcaat ctacaacagc ttttatgtgt attgtaaagg cccctgtcaa agagtgcagc 541 cggggaaact cagggtacag tgcagcacct gcaggcaggc aacgctcacc ttgacccagg 601 gtccatcttg ctgggatgat gttttaattc caaaccggat gagtggtgaa tgccaatccc 661 cacactgccc tgggactagt gcagaatttt tctttaaatg tggagcacac cccacctctg 721 acaaggaaac atcagtagct ttgcacctga tcgcaacaaa tagtcggaac atcacttgca 781 ttacgtgcac agacgtcagg agccccgtcc tggttttcca gtgcaactcc cgccacgtga 841 tttgcttaga ctgtttccac ttatactgtg tgacaagact caatgatcgg cagtttgttc 901 acgaccctca acttggctac tccctgcctt gtgtgggaac tggagacaca gtggtgctta 961 gaggagctct ggggggattc aggagaggag tcgctggctg tcccaactcc ttgattaaag 1021 agctccatca cttcaggatt ctgggagaag agcagtacaa ccggtaccag cagtatggtg 1081 cagaggagtg tgtcctgcag atggggggcg tgttatgccc ccgccctggc tgtggagcgg 1141 ggctgctgcc ggagcctgac cagaggaaag tcacctgcga aggGGGCAAT GGCCTGGGCT 1201 GTGGGTATGG ACAACGAAGA ACAAAATACC CAACTTTCTT GTACAAAGTG GTTGATATCG 1261 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1321 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATCTTACTC 1381 ACATTGAGAT ACATTGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1441 aagatt