Construct: ORF TRCN0000467472
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006700.1_s317c1
- Derived from:
- ccsbBroadEn_10856
- DNA Barcode:
- AGTCTTCAAAGTAGTGTTAGGGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPD2 (2820)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467472
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2820 | GPD2 | glycerol-3-phosphate dehydr... | NM_000408.5 | 51.9% | 51.8% | 30_32delGAT;791G>A;1137_2181delinsA |
| 2 | human | 2820 | GPD2 | glycerol-3-phosphate dehydr... | NM_001083112.3 | 51.9% | 51.8% | 30_32delGAT;791G>A;1137_2181delinsA |
| 3 | human | 2820 | GPD2 | glycerol-3-phosphate dehydr... | XM_005246469.2 | 51.9% | 51.8% | 30_32delGAT;791G>A;1137_2181delinsA |
| 4 | human | 2820 | GPD2 | glycerol-3-phosphate dehydr... | XM_011510977.2 | 51.9% | 51.8% | 30_32delGAT;791G>A;1137_2181delinsA |
| 5 | human | 2820 | GPD2 | glycerol-3-phosphate dehydr... | XM_017003830.1 | 51.9% | 51.8% | 30_32delGAT;791G>A;1137_2181delinsA |
| 6 | human | 2820 | GPD2 | glycerol-3-phosphate dehydr... | XM_024452798.1 | 51.9% | 51.8% | 30_32delGAT;791G>A;1137_2181delinsA |
| 7 | human | 2820 | GPD2 | glycerol-3-phosphate dehydr... | XM_011510978.2 | 47.4% | 47.3% | 0_1ins99;689G>A;1035_2079delinsA |
| 8 | mouse | 14571 | Gpd2 | glycerol phosphate dehydrog... | NM_001145820.1 | 45.1% | 48.2% | (many diffs) |
| 9 | mouse | 14571 | Gpd2 | glycerol phosphate dehydrog... | NM_010274.3 | 45.1% | 48.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1200
- ORF length:
- 1134
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc atttcaaaag gcagtgaaag ggactcttgt tggaggaggt gctcttgcaa 121 ctgttttagg actttctcag tttgctcatt acagaaggaa acaaatgaac ctggcctatg 181 ttaaagcagc agactgcatt tcagaaccag ttaacaggga gcctccttcc agagaagctc 241 agctactgac tttgcaaaac acatctgaat ttgatatcct tgttattgga ggaggagcaa 301 caggaagtgg ctgtgcgcta gatgctgtca ccagaggact aaaaacagcc cttgtagaaa 361 gagatgattt ctcatcaggg accagcagca gaagcactaa attgatccat ggtggtgtga 421 gatatctgca gaaggccatc atgaagttgg atattgagca gtataggatg gtaaaagaag 481 cccttcatga gcgtgccaac ctgctagaaa ttgctcccca tttatcagct ccattgccta 541 taatgcttcc agtttacaag tggtggcagt taccttacta ctgggtagga atcaagctgt 601 atgatttggt tgcaggaagc aattgcctaa aaagcagtta tgtcctcagc aaatcaagag 661 cccttgaaca tttcccaatg ctccagaagg acaaactggt aggagcaatt gtctactatg 721 acggacaaca taacgatgca cggatgaacc ttgccattgc TCTGACTGCT GCCAGGTATG 781 GGGCTGCCAC AGCCAATTAC ATGGAGGTAG TGAGCTTGCT CAAGAAGACA GACCCCCAGA 841 CAGGGAAAGT GCATGTGAGC GGCGCACGGT GCAAGGATGT CCTCACAGGG CAGGAATTTG 901 ACGTGAGAGC CAAATGTGTT ATCAATGCCA CGGGACCTTT CACGGACTCT GTGCGCAAAA 961 TGGATGATAA AGACGCAGCA GCTATCTGCC AGCCAAGTGC TGGTGTCCAT ATTGTGATGC 1021 CTGGTTATTA CAGCCCAGAG AGCATGGGAC TTCTTGACCC AGCGACCAGT GATGGGCGAG 1081 TTATTTTCTT CTTACCCTGG CAAAAGATGA CGATCGCTGG CACTACTGAT ACTCCAACTG 1141 ATGTTACACA CCATCCAATT CCTTCAGAAG AAGATATCAA CTTCATTTTG AATGAAGTAT 1201 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1261 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1321 TTTATATATC TTGTGGAAAG GACGAAGTCT TCAAAGTAGT GTTAGGGATA CGCGTTAAGT 1381 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt