Transcript: Human XM_005246469.2

PREDICTED: Homo sapiens glycerol-3-phosphate dehydrogenase 2 (GPD2), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
GPD2 (2820)
Length:
5495
CDS:
74..2257

Additional Resources:

NCBI RefSeq record:
XM_005246469.2
NBCI Gene record:
GPD2 (2820)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005246469.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000418008 CTACTATGACGGACAACATAA pLKO_005 724 CDS 100% 13.200 18.480 N GPD2 n/a
2 TRCN0000429139 CAGATAGCATGTGTTATTAAA pLKO_005 2640 3UTR 100% 15.000 12.000 N GPD2 n/a
3 TRCN0000435827 ACTGAATTGGGATGATTATAA pLKO_005 1813 CDS 100% 15.000 10.500 N GPD2 n/a
4 TRCN0000414156 ATGGCAGAAGATACCATAAAT pLKO_005 1400 CDS 100% 15.000 10.500 N GPD2 n/a
5 TRCN0000432581 GCAGACCAGAAAGGCTTTATT pLKO_005 1982 CDS 100% 15.000 10.500 N GPD2 n/a
6 TRCN0000220846 GCCATCATGAAGTTGGATATT pLKO.1 446 CDS 100% 13.200 9.240 N GPD2 n/a
7 TRCN0000421802 GCGTGCCAACCTGCTAGAAAT pLKO_005 502 CDS 100% 13.200 9.240 N GPD2 n/a
8 TRCN0000220848 GCAGTTATGTCCTCAGCAAAT pLKO.1 645 CDS 100% 10.800 7.560 N GPD2 n/a
9 TRCN0000220845 CGTGTATTAGAGAGTATCAAT pLKO.1 2021 CDS 100% 5.625 3.938 N GPD2 n/a
10 TRCN0000220847 CCCACACTCTACATTAGGCTT pLKO.1 1502 CDS 100% 2.640 1.848 N GPD2 n/a
11 TRCN0000220849 GCCTTCAGACATTGACAGGTA pLKO.1 1936 CDS 100% 2.640 1.848 N GPD2 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005246469.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10856 pDONR223 100% 51.9% 51.8% None 30_32delGAT;791G>A;1137_2181delinsA n/a
2 ccsbBroad304_10856 pLX_304 0% 51.9% 51.8% V5 30_32delGAT;791G>A;1137_2181delinsA n/a
3 TRCN0000467472 AGTCTTCAAAGTAGTGTTAGGGAT pLX_317 38.9% 51.9% 51.8% V5 30_32delGAT;791G>A;1137_2181delinsA n/a
Download CSV