Construct: ORF TRCN0000467474

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007511.1_s317c1
Derived from:
ccsbBroadEn_06184
DNA Barcode:
TTCATCGATCGAAAAAAATAGAAC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
EXTL3 (2137)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000467474
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2137 EXTL3 exostosin like glycosyltran... NM_001440.4 99.9% 100% 1227G>A
2 human 2137 EXTL3 exostosin like glycosyltran... XM_011544440.3 99.9% 100% 1227G>A
3 human 2137 EXTL3 exostosin like glycosyltran... XM_024447094.1 99.9% 100% 1227G>A
4 human 2137 EXTL3 exostosin like glycosyltran... XM_024447095.1 99.9% 100% 1227G>A
5 human 2137 EXTL3 exostosin like glycosyltran... XM_024447096.1 99.9% 100% 1227G>A
6 human 2137 EXTL3 exostosin like glycosyltran... NR_073468.2 9.5% (many diffs)
7 mouse 54616 Extl3 exostoses (multiple)-like 3 NM_018788.3 88.5% 97.3% (many diffs)
8 mouse 54616 Extl3 exostoses (multiple)-like 3 XM_006519284.2 88.5% 97.3% (many diffs)
9 mouse 54616 Extl3 exostoses (multiple)-like 3 XM_006519285.3 88.5% 97.3% (many diffs)
10 mouse 54616 Extl3 exostoses (multiple)-like 3 XM_006519286.3 88.5% 97.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2823
ORF length:
2757
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgac aggctatacc atgctgcgga atgggggcgc ggggaacgga ggtcagacct
121gcatgctgcg ctggtccaac cgcatccgcc tcacgtggct cagcttcacg ctctttgtca
181tcctggtctt cttcccgctc atcgcccact attacctcac cactctggat gaggctgatg
241aggcaggcaa gcggattttt ggtccccggg tggggaacga gctgtgcgag gtgaagcacg
301tgctggatct gtgccgcatc cgggagtcgg tgagtgaaga gctcctgcag ctggaggcca
361agcgccaaga gctgaacagc gagatcgcca agctgaatct gaagatcgaa gcctgtaaga
421agagcattga gaacgccaag caggacctgc tccagctcaa gaatgtcatc agccagaccg
481agcattccta caaggagctc atggcccaga accagcccaa gctgtccctg cccatccgac
541tgctcccaga gaaggacgat gccggcctcc ctcccccgaa ggccactcgg ggctgccggc
601tacacaactg ctttgattat tctcgttgcc ctctcacctc tggcttcccg gtctacgtct
661atgacagtga ccagtttgtc tttggcagct acctggatcc cttggtcaag caggcttttc
721aggcgacagc acgagctaac gtttatgtta cagaaaatgc agacatcgcc tgcctttacg
781tgatactagt gggagagatg caggagccgg tggtgctgcg gcctgctgag ctggagaagc
841agttgtattc cctgccacac tggcggacgg atggacacaa ccatgtcatc atcaatctgt
901cacgtaagtc agatacacag aaccttctct ataacgtcag tactggccgt gccatggtgg
961cccagtccac cttctacact gtccagtaca gacctggctt tgacttggtc gtatcaccgc
1021tggtccatgc catgtctgag cccaacttca tggaaatccc accacaggtg ccggtgaagc
1081ggaaatatct cttcaccttc cagggcgaga agattgagtc tctgaggtct agccttcagg
1141aggcccgctc cttcgaagag gaaatggagg gcgaccctcc cgccgactac gatgaccgga
1201tcattgccac cctgaaggcg gtgcaggaca gcaagctgga tcaggtcctg gtggaattca
1261cctgcaaaaa ccagcccaaa cccagcctgc caactgagtg ggcactgtgt ggagagcggg
1321aggaccgctt ggaattgctg aagctctcca ccttcgccct catcattacc cccggggacc
1381ctcgcttggt tatttcctct gggtgtgcaa cacggctctt cgaagccctg gaagtcggtg
1441ccgtcccggt ggtgctgggg gagcaggtcc agcttcccta ccaggacatg ctgcagtgga
1501acgaggcggc cctggtggtg ccaaagcctc gtgttaccga ggttcatttc ctgctcagaa
1561gcctctccga tagtgacctc ctggctatga ggcggcaagg ccgctttctc tgggagactt
1621acttctccac tgctgacagt atttttaata ccgtgctggc tatgattagg actcgcatcc
1681agatcccagc cgctcccatc cgggaagagg cggcagctga gatcccccac cgttcaggca
1741aggcggctgg aactgacccc aacatggctg acaacgggga cctggacctg gggccagtgg
1801agacggagcc gccctacgcc tcacccagat acctccgcaa tttcactctg actgtcactg
1861acttttaccg cagctggaac tgtgctccag ggcctttcca tcttttcccc cacactccct
1921ttgaccctgt gttgccctca gaggccaaat tcttgggctc agggactggc tttcggccta
1981ttggtggtgg agctgggggt tctggcaagg aatttcaggc agcgcttgga ggcaatgttc
2041cccgagagca gttcacggtg gtgatgttga cttatgagcg ggaggaagtg cttatgaact
2101ctttagagag gctgaatggc ctcccttacc tgaacaaggt cgtggtggtg tggaattctc
2161ccaagctgcc atcagaggac cttctgtggc ctgacattgg cgtccccatc atggtggtcc
2221gtactgagaa gaacagtttg aacaaccgat tcttaccctg gaatgaaatt gagacagagg
2281ccatcctgtc cattgatgac gatgctcacc tccgccatga cgaaatcatg tttgggttcc
2341gggtgtggag agaagctcgg gaccgcatcg tgggcttccc tggccgttac cacgcatggg
2401acatccccca tcagtcctgg ctctacaact ccaactactc ctgtgagctg tccatggtgc
2461tgacaggtgc tgccttcttt cacaagtatt atgcctacct gtattcttat gtgatgcccc
2521aggccatccg ggacatggtg gatgaataca tcaactgtga ggacattgcc atgaacttcc
2581ttgtctccca catcactcgg aagcccccca tcaaggtgac ctcacggtgg acattccgat
2641gcccaggatg ccctcaggcc ctgtctcatg atgactccca cttccacgag cggcacaagt
2701gcatcaactt cTTCGTGAAG GTGTACGGCT ACATGCCCCT CCTGTACACG CAGTTCAGGG
2761TGGATTCTGT GCTCTTCAAG ACACGCCTGC CCCATGACAA GACCAAGTGC TTCAAGTTCA
2821TCTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC
2881TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT
2941GGCTTTATAT ATCTTGTGGA AAGGACGATT CATCGATCGA AAAAAATAGA ACACGCGTTA
3001AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt

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