Construct: ORF TRCN0000467474
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007511.1_s317c1
- Derived from:
- ccsbBroadEn_06184
- DNA Barcode:
- TTCATCGATCGAAAAAAATAGAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EXTL3 (2137)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467474
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2137 | EXTL3 | exostosin like glycosyltran... | NM_001440.4 | 99.9% | 100% | 1227G>A |
| 2 | human | 2137 | EXTL3 | exostosin like glycosyltran... | XM_011544440.3 | 99.9% | 100% | 1227G>A |
| 3 | human | 2137 | EXTL3 | exostosin like glycosyltran... | XM_024447094.1 | 99.9% | 100% | 1227G>A |
| 4 | human | 2137 | EXTL3 | exostosin like glycosyltran... | XM_024447095.1 | 99.9% | 100% | 1227G>A |
| 5 | human | 2137 | EXTL3 | exostosin like glycosyltran... | XM_024447096.1 | 99.9% | 100% | 1227G>A |
| 6 | human | 2137 | EXTL3 | exostosin like glycosyltran... | NR_073468.2 | 9.5% | (many diffs) | |
| 7 | mouse | 54616 | Extl3 | exostoses (multiple)-like 3 | NM_018788.3 | 88.5% | 97.3% | (many diffs) |
| 8 | mouse | 54616 | Extl3 | exostoses (multiple)-like 3 | XM_006519284.2 | 88.5% | 97.3% | (many diffs) |
| 9 | mouse | 54616 | Extl3 | exostoses (multiple)-like 3 | XM_006519285.3 | 88.5% | 97.3% | (many diffs) |
| 10 | mouse | 54616 | Extl3 | exostoses (multiple)-like 3 | XM_006519286.3 | 88.5% | 97.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2823
- ORF length:
- 2757
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac aggctatacc atgctgcgga atgggggcgc ggggaacgga ggtcagacct 121 gcatgctgcg ctggtccaac cgcatccgcc tcacgtggct cagcttcacg ctctttgtca 181 tcctggtctt cttcccgctc atcgcccact attacctcac cactctggat gaggctgatg 241 aggcaggcaa gcggattttt ggtccccggg tggggaacga gctgtgcgag gtgaagcacg 301 tgctggatct gtgccgcatc cgggagtcgg tgagtgaaga gctcctgcag ctggaggcca 361 agcgccaaga gctgaacagc gagatcgcca agctgaatct gaagatcgaa gcctgtaaga 421 agagcattga gaacgccaag caggacctgc tccagctcaa gaatgtcatc agccagaccg 481 agcattccta caaggagctc atggcccaga accagcccaa gctgtccctg cccatccgac 541 tgctcccaga gaaggacgat gccggcctcc ctcccccgaa ggccactcgg ggctgccggc 601 tacacaactg ctttgattat tctcgttgcc ctctcacctc tggcttcccg gtctacgtct 661 atgacagtga ccagtttgtc tttggcagct acctggatcc cttggtcaag caggcttttc 721 aggcgacagc acgagctaac gtttatgtta cagaaaatgc agacatcgcc tgcctttacg 781 tgatactagt gggagagatg caggagccgg tggtgctgcg gcctgctgag ctggagaagc 841 agttgtattc cctgccacac tggcggacgg atggacacaa ccatgtcatc atcaatctgt 901 cacgtaagtc agatacacag aaccttctct ataacgtcag tactggccgt gccatggtgg 961 cccagtccac cttctacact gtccagtaca gacctggctt tgacttggtc gtatcaccgc 1021 tggtccatgc catgtctgag cccaacttca tggaaatccc accacaggtg ccggtgaagc 1081 ggaaatatct cttcaccttc cagggcgaga agattgagtc tctgaggtct agccttcagg 1141 aggcccgctc cttcgaagag gaaatggagg gcgaccctcc cgccgactac gatgaccgga 1201 tcattgccac cctgaaggcg gtgcaggaca gcaagctgga tcaggtcctg gtggaattca 1261 cctgcaaaaa ccagcccaaa cccagcctgc caactgagtg ggcactgtgt ggagagcggg 1321 aggaccgctt ggaattgctg aagctctcca ccttcgccct catcattacc cccggggacc 1381 ctcgcttggt tatttcctct gggtgtgcaa cacggctctt cgaagccctg gaagtcggtg 1441 ccgtcccggt ggtgctgggg gagcaggtcc agcttcccta ccaggacatg ctgcagtgga 1501 acgaggcggc cctggtggtg ccaaagcctc gtgttaccga ggttcatttc ctgctcagaa 1561 gcctctccga tagtgacctc ctggctatga ggcggcaagg ccgctttctc tgggagactt 1621 acttctccac tgctgacagt atttttaata ccgtgctggc tatgattagg actcgcatcc 1681 agatcccagc cgctcccatc cgggaagagg cggcagctga gatcccccac cgttcaggca 1741 aggcggctgg aactgacccc aacatggctg acaacgggga cctggacctg gggccagtgg 1801 agacggagcc gccctacgcc tcacccagat acctccgcaa tttcactctg actgtcactg 1861 acttttaccg cagctggaac tgtgctccag ggcctttcca tcttttcccc cacactccct 1921 ttgaccctgt gttgccctca gaggccaaat tcttgggctc agggactggc tttcggccta 1981 ttggtggtgg agctgggggt tctggcaagg aatttcaggc agcgcttgga ggcaatgttc 2041 cccgagagca gttcacggtg gtgatgttga cttatgagcg ggaggaagtg cttatgaact 2101 ctttagagag gctgaatggc ctcccttacc tgaacaaggt cgtggtggtg tggaattctc 2161 ccaagctgcc atcagaggac cttctgtggc ctgacattgg cgtccccatc atggtggtcc 2221 gtactgagaa gaacagtttg aacaaccgat tcttaccctg gaatgaaatt gagacagagg 2281 ccatcctgtc cattgatgac gatgctcacc tccgccatga cgaaatcatg tttgggttcc 2341 gggtgtggag agaagctcgg gaccgcatcg tgggcttccc tggccgttac cacgcatggg 2401 acatccccca tcagtcctgg ctctacaact ccaactactc ctgtgagctg tccatggtgc 2461 tgacaggtgc tgccttcttt cacaagtatt atgcctacct gtattcttat gtgatgcccc 2521 aggccatccg ggacatggtg gatgaataca tcaactgtga ggacattgcc atgaacttcc 2581 ttgtctccca catcactcgg aagcccccca tcaaggtgac ctcacggtgg acattccgat 2641 gcccaggatg ccctcaggcc ctgtctcatg atgactccca cttccacgag cggcacaagt 2701 gcatcaactt cTTCGTGAAG GTGTACGGCT ACATGCCCCT CCTGTACACG CAGTTCAGGG 2761 TGGATTCTGT GCTCTTCAAG ACACGCCTGC CCCATGACAA GACCAAGTGC TTCAAGTTCA 2821 TCTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 2881 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 2941 GGCTTTATAT ATCTTGTGGA AAGGACGATT CATCGATCGA AAAAAATAGA ACACGCGTTA 3001 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt