Transcript: Mouse XM_006519284.2

PREDICTED: Mus musculus exostoses (multiple)-like 3 (Extl3), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Extl3 (54616)
Length:
5958
CDS:
645..3404

Additional Resources:

NCBI RefSeq record:
XM_006519284.2
NBCI Gene record:
Extl3 (54616)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006519284.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000141339 CCGTACTGAGAAGAACAGTTT pLKO.1 2798 CDS 100% 4.950 6.930 N EXTL3 n/a
2 TRCN0000323006 CCGTACTGAGAAGAACAGTTT pLKO_005 2798 CDS 100% 4.950 6.930 N EXTL3 n/a
3 TRCN0000093747 GCCATGATTAGGACTCGAATT pLKO.1 2238 CDS 100% 0.000 0.000 N Extl3 n/a
4 TRCN0000316739 GCCATGATTAGGACTCGAATT pLKO_005 2238 CDS 100% 0.000 0.000 N Extl3 n/a
5 TRCN0000375769 ACACTGAGGACTGTTCATAAG pLKO_005 3641 3UTR 100% 10.800 7.560 N Extl3 n/a
6 TRCN0000142093 GCTGCCTTCTTTCACAAGTAT pLKO.1 3048 CDS 100% 5.625 3.938 N EXTL3 n/a
7 TRCN0000141559 CAAGACCAAGTGCTTCAAGTT pLKO.1 3377 CDS 100% 4.950 3.465 N EXTL3 n/a
8 TRCN0000323007 CAAGACCAAGTGCTTCAAGTT pLKO_005 3377 CDS 100% 4.950 3.465 N EXTL3 n/a
9 TRCN0000093745 GCGGAAATATCTCTTCACTTT pLKO.1 1658 CDS 100% 4.950 3.465 N Extl3 n/a
10 TRCN0000316673 GCGGAAATATCTCTTCACTTT pLKO_005 1658 CDS 100% 4.950 3.465 N Extl3 n/a
11 TRCN0000093746 GCGGCACAAGTGTATCAACTT pLKO.1 3269 CDS 100% 4.950 3.465 N Extl3 n/a
12 TRCN0000316670 GCGGCACAAGTGTATCAACTT pLKO_005 3269 CDS 100% 4.950 3.465 N Extl3 n/a
13 TRCN0000093748 GCCTTCTTTCACAAGTATTAT pLKO.1 3051 CDS 100% 15.000 9.000 N Extl3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006519284.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06184 pDONR223 100% 88.5% 97.3% None (many diffs) n/a
2 ccsbBroad304_06184 pLX_304 0% 88.5% 97.3% V5 (many diffs) n/a
3 TRCN0000467474 TTCATCGATCGAAAAAAATAGAAC pLX_317 4.3% 88.5% 97.3% V5 (many diffs) n/a
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