Construct: ORF TRCN0000467481
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015940.1_s317c1
- Derived from:
- ccsbBroadEn_16134
- DNA Barcode:
- CCATACTTCGACACTAGACACCGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZDHHC20 (253832)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467481
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 253832 | ZDHHC20 | zinc finger DHHC-type conta... | NM_153251.3 | 35.9% | 35% | (many diffs) |
| 2 | human | 253832 | ZDHHC20 | zinc finger DHHC-type conta... | XM_006719797.1 | 35.8% | 34.9% | (many diffs) |
| 3 | human | 253832 | ZDHHC20 | zinc finger DHHC-type conta... | XM_006719796.1 | 34.9% | 34% | (many diffs) |
| 4 | human | 253832 | ZDHHC20 | zinc finger DHHC-type conta... | NM_001330059.2 | 34.8% | 33.9% | (many diffs) |
| 5 | human | 253832 | ZDHHC20 | zinc finger DHHC-type conta... | NM_001286638.1 | 18.1% | 17.1% | (many diffs) |
| 6 | human | 253832 | ZDHHC20 | zinc finger DHHC-type conta... | NR_104487.2 | 7.1% | (many diffs) | |
| 7 | human | 253832 | ZDHHC20 | zinc finger DHHC-type conta... | XR_001749530.1 | 7.1% | (many diffs) | |
| 8 | human | 253832 | ZDHHC20 | zinc finger DHHC-type conta... | XR_429215.1 | 7% | (many diffs) | |
| 9 | human | 253832 | ZDHHC20 | zinc finger DHHC-type conta... | XR_001749531.1 | 7% | (many diffs) | |
| 10 | human | 253832 | ZDHHC20 | zinc finger DHHC-type conta... | NR_104486.2 | 7% | 1_132del;523_5518del | |
| 11 | human | 253832 | ZDHHC20 | zinc finger DHHC-type conta... | XR_941546.1 | 6.8% | (many diffs) | |
| 12 | mouse | 75965 | Zdhhc20 | zinc finger, DHHC domain co... | NM_029492.4 | 30.8% | 31.5% | (many diffs) |
| 13 | mouse | 75965 | Zdhhc20 | zinc finger, DHHC domain co... | XM_006519661.3 | 25.1% | 25.6% | (many diffs) |
| 14 | mouse | 75965 | Zdhhc20 | zinc finger, DHHC domain co... | XM_006519660.3 | 24.4% | 24.9% | (many diffs) |
| 15 | mouse | 75965 | Zdhhc20 | zinc finger, DHHC domain co... | XM_006519659.3 | 24.4% | 24.8% | (many diffs) |
| 16 | mouse | 75965 | Zdhhc20 | zinc finger, DHHC domain co... | XM_006519657.3 | 23.9% | 24.4% | (many diffs) |
| 17 | mouse | 75965 | Zdhhc20 | zinc finger, DHHC domain co... | XM_006519658.3 | 23.9% | 24.4% | (many diffs) |
| 18 | mouse | 75965 | Zdhhc20 | zinc finger, DHHC domain co... | XR_001781166.1 | 6% | (many diffs) | |
| 19 | mouse | 75965 | Zdhhc20 | zinc finger, DHHC domain co... | XR_001781165.1 | 6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 456
- ORF length:
- 390
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gccctggacg ctgtggcgct gctgccagcg cgtcgtgggc tgggtgccgg 121 tgctcttcat caccttcgtg gtcgtctggt ccTACTACGC GTACGTGGTG GAGCTCTGCG 181 TGTTTACTAT TTTTGGAAAT GAAGAAAATG GAAAGACCGT TGTTTACCTT GTGGCTTTCC 241 ATCTGTTCTT TGTTATGTTT GTATGGTCCT ATTGGATGAC AATTTTCACA TCTCCCGCTT 301 CCCCCTCCAA AGAGTTCTAC TTGTCCAATT CTGAAAAGGA ACGTTATGAA AAAGAATTCA 361 GCCAAGAAAG ACAACAAGAA ATTTTGAGAA GAGCAGCAAG AGCTTTACCT ATCTATACCA 421 CATCAGCTTC AAAAAGCCTC ATGCAGTCTA CAAAATACCC AACTTTCTTG TACAAAGTGG 481 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 541 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 601 ACCATACTTC GACACTAGAC ACCGTACGCG TTAAGTCgac aatcaacctc tggattacaa 661 aatttgtgaa agatt