Construct: ORF TRCN0000467498
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008182.1_s317c1
- Derived from:
- ccsbBroadEn_06585
- DNA Barcode:
- CCTCGACGATCTGCGTACGAATCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MLN (4295)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467498
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4295 | MLN | motilin | NM_002418.3 | 99.7% | 99.1% | 44T>C |
| 2 | human | 4295 | MLN | motilin | NM_001040109.2 | 98.8% | 97.3% | 44T>C;336_337insGCA |
| 3 | human | 4295 | MLN | motilin | NM_001184698.2 | 93.6% | 90.4% | 44T>C;316_317ins21 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 414
- ORF length:
- 345
- Sequence:
-
1 tcttccattT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCACCAT GGTATCCCGT AAGGCTGTGG CTGCTCTGCT GGTGGTGCAT GCAGCTGCCA 121 TGCTGGCCTC CCAGACGGAA GCCTTCGTCC CCATCTTCAC CTATGGCGAA CTCCAGAGGA 181 TGCAGGAAAA GGAACGGAAT AAAGGGCAAA AGAAATCCCT GAGTGTATGG CAGAGGTCTG 241 GGGAGGAAGG TCCTGTAGAC CCTGCGGAGC CCATCAGGGA AGAAGAAAAC GAAATGATCA 301 AGCTGACTGC TCCTCTGGAA ATTGGAATGA GGATGAACTC CAGACAGCTG GAAAAGTACC 361 CGGCCACCCT GGAAGGGCTG CTGAGTGAGA TGCTTCCCCA GCATGCAGCC AAGTTGCCAA 421 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 481 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 541 TATCTTGTGG AAAGGACGAC CTCGACGATC TGCGTACGAA TCAACGCGTT AAGTCgacaa 601 tcaacctctg gattacaaaa tttgtgaaag att