Construct: ORF TRCN0000467503
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004435.1_s317c1
- Derived from:
- ccsbBroadEn_05299
- DNA Barcode:
- CGCAGATAACCCTCGATAACGGCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CNIH2 (254263)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467503
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 254263 | CNIH2 | cornichon family AMPA recep... | NM_182553.3 | 100% | 100% | |
| 2 | human | 254263 | CNIH2 | cornichon family AMPA recep... | NR_073079.2 | 34.6% | 1_282del;359_360insGCAC;759_1370del | |
| 3 | human | 254263 | CNIH2 | cornichon family AMPA recep... | NR_073078.2 | 34.2% | 1_282del;433_458del;789_1400del | |
| 4 | mouse | 12794 | Cnih2 | cornichon family AMPA recep... | NM_009920.4 | 97.2% | 100% | (many diffs) |
| 5 | mouse | 12794 | Cnih2 | cornichon family AMPA recep... | NM_001302333.1 | 69.3% | 68.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 546
- ORF length:
- 480
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gttcaccttc gccgcgttct gctacatgct caccctggtg ctgtgcgcct 121 ccctcatctt ctttgtcatc tggcacatca tagcctttga tgagctgcgg accgacttca 181 agaaccccat cgaccaggGG AACCCTGCGC GGGCACGCGA GCGTTTAAAA AACATCGAAC 241 GCATCTGCTG CCTCCTGAGG AAGCTGGTGG TCCCAGAATA CTCCATCCAC GGCCTCTTCT 301 GTCTGATGTT TCTGTGTGCA GCAGAGTGGG TGACCCTGGG CCTCAACATC CCCCTCCTCT 361 TCTACCACCT CTGGAGGTAC TTCCACCGTC CTGCAGATGG CTCTGAGGTC ATGTATGATG 421 CGGTCTCCAT CATGAATGCT GACATTCTCA ACTACTGCCA GAAGGAGTCC TGGTGCAAAC 481 TTGCCTTCTA CCTGCTCTCC TTCTTCTATT ACCTGTACAG TATGGTTTAT ACGTTGGTGA 541 GTTTCTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 601 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 661 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACGCAGATAA CCCTCGATAA CGGCTACGCG 721 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt