Construct: ORF TRCN0000467515
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004070.1_s317c1
- Derived from:
- ccsbBroadEn_13241
- DNA Barcode:
- TAATACGATGCACAGACCATATCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KLC3 (147700)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467515
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 147700 | KLC3 | kinesin light chain 3 | NM_177417.3 | 99.8% | 99.8% | 487_489delCAG |
| 2 | human | 147700 | KLC3 | kinesin light chain 3 | XM_024451371.1 | 90.6% | 90.6% | 1_153del;640_642delCAG |
| 3 | human | 147700 | KLC3 | kinesin light chain 3 | XM_024451370.1 | 77.1% | 73% | 487_489delCAG;1441_1708del;1781_1956del |
| 4 | human | 147700 | KLC3 | kinesin light chain 3 | XM_024451369.1 | 71.5% | 67.8% | (many diffs) |
| 5 | mouse | 232943 | Klc3 | kinesin light chain 3 | NM_001286038.1 | 85.3% | 90.1% | (many diffs) |
| 6 | mouse | 232943 | Klc3 | kinesin light chain 3 | NM_001286039.1 | 85.3% | 90.1% | (many diffs) |
| 7 | mouse | 232943 | Klc3 | kinesin light chain 3 | NM_146182.4 | 85.3% | 90.1% | (many diffs) |
| 8 | mouse | 232943 | Klc3 | kinesin light chain 3 | XM_017322154.1 | 77.6% | 81% | (many diffs) |
| 9 | mouse | 232943 | Klc3 | kinesin light chain 3 | XM_017322153.1 | 76.9% | 80.3% | (many diffs) |
| 10 | mouse | 232943 | Klc3 | kinesin light chain 3 | XM_006539826.3 | 67.6% | 71.4% | (many diffs) |
| 11 | mouse | 232943 | Klc3 | kinesin light chain 3 | XM_006539823.3 | 62.7% | 65.5% | (many diffs) |
| 12 | mouse | 232943 | Klc3 | kinesin light chain 3 | XM_017322152.1 | 60% | 61% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1575
- ORF length:
- 1509
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tgtgcaggta gcggctcctg gaagtgcagg gctgggccca gagcgcctga 121 gccctgagga gctggtgcgg cagacgcggc aagtggtcca ggggctggag gcgctgcggg 181 cagagcacca tggcctggct gggcacctgg cggaggccct ggcgggacag ggcccggcag 241 ccggcttgga gatgctggag gaaaagcagc aggtggtgag ccactcgctg gaggccatcg 301 agctggggct gggcgaggcc caggtgctgc tggccctgtc ggcacatgtg ggtgcactgg 361 aggcagagaa gcagcggctg cgctcgcagg cccggcggct ggcccaggag aacgtgtggc 421 tgcgggagga actggaggag acgcagcggc ggcttcgggc cagcgaggag tccgtggccc 481 agctggagga ggagaagcgc cacctggagt tcctggggca gctgcgacag tacgacccac 541 cggcggagag ccagtctgag tccccgcctc gccgagacag cctggcctcc ctgttcccca 601 gcgaggagga ggagaggaaa ggtcctgagg ccgcaggagc agcagctgct cagcagggtg 661 gctatgagat ccctgcccgc cttcggaccc tgcataacct cgtgatccag tacgcggggc 721 agggccgcta tgaggtggcg gtgcctctgt gccgccaggc cttggaggac ctggagcgca 781 gctcgggcca ctgccaccct gacgtggcca ccatgctcaa catcctggcg ctggtgtacc 841 gggaccagaa caagtacaaa gaagccacag accttctcca tgatgccctg cagatccggg 901 agcagacgct gggccctgag caccccgcgg tggccgccac gctcaacaac ttggctgtcc 961 tctatgggaa gcgtgggcgt taccgggagg cagagcccct gtgccagcgc gctttggaga 1021 tccgagagaa ggtcctgggt gctgaccacc cagatgtggc caagcagctc aacaacctgg 1081 ccctgctgtg ccagaaccag ggcaagtttg aggacgtgga gcggcactat gcccgggccc 1141 tgagcatcta tgaggcactg ggcgggcccc atgaccccaa cgtggccaag accaagaaca 1201 acctggcctc agcctacctg aaacagaaca agtatcaaca agcggaagag ctgtacaaag 1261 aaaTCCTCCA CAAGGAGGAC CTACCCGCCC CTCTCGGTGC CCCCAACACA GGCACAGCTG 1321 GTGACGCAGA ACAGGCCCTT CGCCGCAGCA GCTCACTCTC CAAGATCCGT GAGTCTATCA 1381 GGCGAGGAAG TGAGAAGCTG GTCTCCCGGC TCCGAGGCGA GGCGGCGGCA GGAGCAGCCG 1441 GAATGAAGAG AGCCATGTCA CTCAACACAC TGAACGTGGA TGCTCCAAGG GCTCCTGGGA 1501 CTCAGTTTCC CAGCTGGCAC CTGGACAAGG CCCCTCGGAC CCTCAGCGCC AGCACCCAGG 1561 ACCTGAGCCC CCACTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1621 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1681 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TAATACGATG CACAGACCAT 1741 ATCGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt