Construct: ORF TRCN0000467516
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013274.1_s317c1
- Derived from:
- ccsbBroadEn_12432
- DNA Barcode:
- CAGGTCTCGCGGGTCGCGTGAAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- THADA (63892)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467516
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 63892 | THADA | THADA armadillo repeat cont... | NM_001271644.2 | 100% | 100% | |
2 | human | 63892 | THADA | THADA armadillo repeat cont... | NM_001271643.2 | 95.3% | 95% | (many diffs) |
3 | human | 63892 | THADA | THADA armadillo repeat cont... | XM_011533015.3 | 69.5% | 69.3% | (many diffs) |
4 | human | 63892 | THADA | THADA armadillo repeat cont... | XM_006712068.3 | 61.4% | 61.3% | (many diffs) |
5 | human | 63892 | THADA | THADA armadillo repeat cont... | XM_006712065.1 | 47.5% | 47.4% | (many diffs) |
6 | human | 63892 | THADA | THADA armadillo repeat cont... | XM_006712064.2 | 46.7% | 46.6% | (many diffs) |
7 | human | 63892 | THADA | THADA armadillo repeat cont... | XM_006712067.1 | 46.6% | 42.9% | (many diffs) |
8 | human | 63892 | THADA | THADA armadillo repeat cont... | NM_001345923.1 | 45.7% | 45.6% | (many diffs) |
9 | human | 63892 | THADA | THADA armadillo repeat cont... | NM_001083953.1 | 45.7% | 45.6% | (many diffs) |
10 | human | 63892 | THADA | THADA armadillo repeat cont... | NM_001345925.1 | 45.7% | 45.6% | (many diffs) |
11 | human | 63892 | THADA | THADA armadillo repeat cont... | NM_022065.4 | 45.7% | 45.6% | (many diffs) |
12 | human | 63892 | THADA | THADA armadillo repeat cont... | XM_006712066.1 | 45.3% | 43.1% | (many diffs) |
13 | human | 63892 | THADA | THADA armadillo repeat cont... | NR_073394.1 | 44.8% | (many diffs) | |
14 | human | 63892 | THADA | THADA armadillo repeat cont... | NR_144316.1 | 44.6% | (many diffs) | |
15 | human | 63892 | THADA | THADA armadillo repeat cont... | NM_001345924.1 | 43.7% | 43.5% | (many diffs) |
16 | human | 63892 | THADA | THADA armadillo repeat cont... | XM_006712063.1 | 43.6% | 43.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2748
- ORF length:
- 2682
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg tgtaaagaag aagaaagaaa tgcaagttgc tgcgctgacc atttgccatc 121 aggaccttga aactttgaaa tcttttgctg atgtggaagg gaaaaatcta gcttctttgc 181 tgttacattg tgtgcaactc acggatggag tgtcacaaat ccattatatt aaacagattg 241 tgcctctgct ggagaaagca gataaaaatg gcatgtgtga tcccactatt caaagttgtt 301 tggatatctt agcaggcatt tatctttctt tgagtctaaa gaatcccttg aagaaagtat 361 tggcaagctc actaaatagc ctgcctgatt tttttctacc tgaggctatg caccgtttta 421 cttctcgtct tcaggaagaa ttgaatacta ctgacttata ctcttacagg aaagttactg 481 acaatatttc ttcctgtatg gagaacttta acttgggtag agcaagtgtt aataatctgc 541 ttaaaaatgt gcttcatttt ctgcagaaga gtttaattga aatcctggaa gaaaatagaa 601 aatgtgctgg aaatcatatt attcaaacac agttgatgaa tgacttactg gtaggcatta 661 gagtttcaat gatgttagta cagaaagtac aagatttcca gggaaatctt tggaagactt 721 ccgattctcc catatggcaa aatatgtgtg gattgctgag tatttttacc aaggttttaa 781 gcgatgatga tctgttacag actgtacaga gcacatctgg attagctatt attcttttta 841 ttaagactat gtttcacccg tctgaaaaga ttcctcattt gattagcagt gtgctgcttc 901 gttcagtgga ctgcaccagt gtccccgagt ggtttatgag cagctgcagg agcctctgtt 961 gtggtgacat ctctcagtca gctgtcttat tcctctgtca ggggacactt gccatgttgg 1021 actggcagaa cggaagcatg ggtcggagtg gggaggccct gctcttggat actgcacatg 1081 ttttgttcac cttgagttca cagattaaag agccaacgct ggaaatgttt ctgtctagaa 1141 tcttagcatc ctggactaat tcagccatac aagtccttga atcaagttcc ccgagcctaa 1201 cggacagcct gaatgggaat tcaagtatag ttgggagact tttggaatat gtctataccc 1261 attgggaaca tccattggat gctctgagac accaaaccaa aatcatgttc aaaaaccttc 1321 tccaaatgca ccggctcact gtggaaggtg cagatttcgt ccctgatcct ttctttgtgg 1381 aattgactga gagtctttta cgattggaat ggcatattaa aggaaagtac acgtgccttg 1441 gttgtttggt agagtgcata ggagttgaac atattttggc tatagataaa actattccat 1501 ctcaaatctt agaggtgatg ggagaccagt cattggtacc ttatgcaagt gacctcttgg 1561 aaaccatgtt tagaaatcat aagagtcatt tgaaatccca gactgctgag agttcttgga 1621 ttgaccagtg gcatgagact tgggtttctc ctctcctttt tatattgtgt gaaggaaact 1681 tggatcaaaa atcttacgtg attgattatt acttgccaaa attattaagt tacagccctg 1741 aaagcttaca gtacatggta aagattcttc agacttctat tgatgctaaa actggacaag 1801 agcaatcttt cccatcctta gggtcttgta atagcagggg ggctctggga gctttgatgg 1861 catgtctgcg aatagctaga gctcatggac atcttcagtc tgcaactgat acctgggaga 1921 acctcgtgtc tgatgcaaga ataaagcaag gcttaattca tcagcattgc caagtaagga 1981 tagatacatt aggcttgctt tgtgaaagta atcggagcac agaaattgtt tccatggaag 2041 aaatgcagtg gattcagttc tttattacat acaatcttaa cagccagtct ccaggagtgc 2101 ggcaacagat ctgttctctt cttaaaaagt tgttttgtag gatacaggaa agttctcagg 2161 tactttataa attggagcag agtaaatcca aacgtgaacc agagaatgag ttaaccaaac 2221 agcacccttc tgtttcttta cagcagtata agaatttcat gtcatccatt tgtaacagtc 2281 tttttgaagc attgtttcct ggatcttcct actcgactag attttcagct ttaaccattt 2341 taggttcaat agctgaagtt tttcatgtcc cagaaggcag aatttataca gtatatcagc 2401 tgagtcatga tattGATGTT GGTCGTTTCC AAACACTAAT GGAATGTTTT ACCAGCACTT 2461 TTGAAGACGT GAAAATTTTA GCATTTGATC TTCTGATGAA GTTATCAAAA ACAGCTGTAC 2521 ATTTTCAGGA TTCGGGGAAA CTGCAAGGCT TATTTCAGGC AGCATTGGAG CTCAGCACAA 2581 GCACCAAACC ATACGACTGT GTGACAGCTT CCTACCTGCT GAACTTCTTA ATCTGGCAGG 2641 ATGCTCTACC GTCATCCTTG TCTGCCTACT TAACTCAGCA AGTTGCATGT GATAATGGAG 2701 ATAGGCCTGC TGCTGTGGTG GAAAGGAACA CATTAATGGG TTTGTATTGC CCAACTTTCT 2761 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 2821 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 2881 GTGGAAAGGA CGACAGGTCT CGCGGGTCGC GTGAAGGACG CGTTAAGTCg acaatcaacc 2941 tctggattac aaaatttgtg aaagatt