Construct: ORF TRCN0000467524
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002135.1_s317c1
- Derived from:
- ccsbBroadEn_02150
- DNA Barcode:
- GTTTTCCGTGTACAAGGTCCCCTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RAB9A (9367)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467524
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9367 | RAB9A | RAB9A, member RAS oncogene ... | NM_001195328.1 | 100% | 100% | |
| 2 | human | 9367 | RAB9A | RAB9A, member RAS oncogene ... | NM_004251.5 | 100% | 100% | |
| 3 | human | 9366 | RAB9BP1 | RAB9B, member RAS oncogene ... | NR_000039.2 | 85.8% | (many diffs) | |
| 4 | mouse | 56382 | Rab9 | RAB9, member RAS oncogene f... | NM_019773.2 | 90.3% | 95.5% | (many diffs) |
| 5 | mouse | 56382 | Rab9 | RAB9, member RAS oncogene f... | XM_006528922.1 | 90.3% | 95.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 669
- ORF length:
- 603
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc aggaaaatca tcacttttta aagtaattct ccttggagat ggtggagttg 121 ggaagagttc acttatgaac agatatgtaa ctaataagtt tgatacccag ctcttccata 181 caataggtgt ggaattttta aataaagatt tggaagtgga tggacatttt gttaccatgc 241 agatttGGGA CACGGCAGGT CAGGAGCGAT TCCGAAGCCT GAGGACACCA TTTTACAGAG 301 GTTCTGACTG CTGCCTGCTT ACTTTTAGTG TCGATGATTC ACAAAGCTTC CAGAACTTAA 361 GTAACTGGAA GAAAGAATTC ATATATTATG CAGATGTGAA AGAGCCTGAG AGCTTTCCTT 421 TTGTGATTCT GGGTAACAAG ATTGACATAA GCGAACGGCA GGTGTCTACA GAAGAAGCCC 481 AAGCTTGGTG CAGGGACAAC GGCGACTATC CTTATTTTGA AACAAGTGCA AAAGATGCCA 541 CAAATGTGGC AGCAGCCTTT GAGGAAGCGG TTCGAAGAGT TCTTGCTACC GAGGATAGGT 601 CAGATCATTT GATTCAGACA GACACAGTCA ATCTTCACCG AAAGCCCAAG CCTAGCTCAT 661 CTTGCTGTTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 721 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 781 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGTTTTC CGTGTACAAG GTCCCCTGAC 841 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt