Construct: ORF TRCN0000467527
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013420.1_s317c1
- Derived from:
- ccsbBroadEn_10327
- DNA Barcode:
- TCTGGTGTTCCCCGCCCAAACTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TOB2P1 (222699)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467527
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10766 | TOB2 | transducer of ERBB2, 2 | NM_016272.4 | 31.1% | 23.9% | (many diffs) |
| 2 | human | 10766 | TOB2 | transducer of ERBB2, 2 | XM_005261315.2 | 31.1% | 23.9% | (many diffs) |
| 3 | human | 10766 | TOB2 | transducer of ERBB2, 2 | XM_006724105.3 | 31.1% | 23.9% | (many diffs) |
| 4 | human | 10766 | TOB2 | transducer of ERBB2, 2 | XM_017028539.1 | 31.1% | 23.9% | (many diffs) |
| 5 | human | 222699 | TOB2P1 | transducer of ERBB2, 2 pseu... | NR_002936.2 | 10.2% | 1_438del;808_3592del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 438
- ORF length:
- 369
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttGGCACCAT GGTGGACCCC GTTGGGGAGC TGGCCGCTAA GCGAAGTGGC CTGACAGTGG 121 AAGATGTGCG GGCCAATGTG CCTGAGGAGC TGAGCATATG GATTGACCCC TTCGGGGTGT 181 CCTACCAGAT TGGTGAGAAG GGAGCAGTGA AAGTGCTGTA CCTGGATGAC AGTGACGGCT 241 GTGGGGCCCC GGAGCTGGAC ATGAAGATCA AGAGCAGTTT CACTCCTGAC GACCAGATGC 301 TCTTTCTCCT CGGAAGCCAG GACAGCTCCT TGTCCAACTC CCCGTCGCTA TGCTTTGCCC 361 ATCACCCAGC CCCACCTTCA TCCTCTGCCT CGCCCAGCCC ATCACCTTCA CCATGGACTC 421 CTTTGCTGCC ACCAAATTTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 481 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 541 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATCTGGTG TTCCCCGCCC 601 AAACTCAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt