Transcript: Human XM_017028539.1

PREDICTED: Homo sapiens transducer of ERBB2, 2 (TOB2), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
TOB2 (10766)
Length:
6854
CDS:
3001..4035

Additional Resources:

NCBI RefSeq record:
XM_017028539.1
NBCI Gene record:
TOB2 (10766)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017028539.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000265689 TGGGTCGCAAGTCCTTATTTA pLKO_005 4934 3UTR 100% 15.000 21.000 N TOB2 n/a
2 TRCN0000255821 TTGACATGGCCCAGGTATTTG pLKO_005 3896 CDS 100% 13.200 18.480 N TOB2 n/a
3 TRCN0000004167 CGGCCTGTGATTTATTTGGTT pLKO.1 4243 3UTR 100% 3.000 2.400 N TOB2 n/a
4 TRCN0000255820 TTGAGGTGTCCTACCAGATTG pLKO_005 3290 CDS 100% 10.800 7.560 N TOB2 n/a
5 TRCN0000004165 GAGCTGAGTGTCTGGATTGAT pLKO.1 3265 CDS 100% 5.625 3.938 N TOB2 n/a
6 TRCN0000004166 AGAGCTGGACAAGGAGATCAA pLKO.1 3369 CDS 100% 4.950 3.465 N TOB2 n/a
7 TRCN0000004164 CTGGCTTCCGCTGTGTTCACA pLKO.1 3158 CDS 100% 1.000 0.700 N TOB2 n/a
8 TRCN0000255823 TCCTTCGCTGCCACCAAATTT pLKO_005 3538 CDS 100% 15.000 9.000 N TOB2 n/a
9 TRCN0000255822 TCCTGGAGAAGACACCCTTTG pLKO_005 3938 CDS 100% 6.000 3.600 N TOB2 n/a
10 TRCN0000313176 GCCTCAGCTACAACCTGAATA pLKO_005 3965 CDS 100% 13.200 7.920 N Tob2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017028539.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10327 pDONR223 100% 31.1% 23.9% None (many diffs) n/a
2 ccsbBroad304_10327 pLX_304 0% 31.1% 23.9% V5 (many diffs) n/a
3 TRCN0000467527 TCTGGTGTTCCCCGCCCAAACTCA pLX_317 98.4% 31.1% 23.9% V5 (many diffs) n/a
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