Construct: ORF TRCN0000467534
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003396.1_s317c1
- Derived from:
- ccsbBroadEn_00416
- DNA Barcode:
- CCTAACCTGGAGCCGCCGGTATAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CYP27A1 (1593)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467534
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1593 | CYP27A1 | cytochrome P450 family 27 s... | NM_000784.4 | 100% | 100% | |
| 2 | human | 1593 | CYP27A1 | cytochrome P450 family 27 s... | XM_017003488.2 | 73.6% | 71.5% | 0_1ins229;26_27ins191 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1659
- ORF length:
- 1593
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgcgctgggc tgcgcgaggc tgaggtgggc gctgcgaggg gccggccgtg 121 gcctctgccc ccacggggcc agagccaagg ccgcgatccc tgccgccctc ccctcggaca 181 aggccaccgg agctcccgga gccgggcctg gtgtccggcg gcggcaacgg agcttagagg 241 agattccacg tctaggacag ctgcgcttct tctttcagct gttcgttcaa ggctatgccc 301 tgcaactgca ccagttacag gtgctttaca aggccaagta cggtccaatg tggatgtcct 361 acttagggcc tcagatgcac gtgaacctgg ccagtgcccc gctcttggag caagtgatgc 421 ggcaagaggg caagtaccca gtacggaacg acatggagct atggaaggag caccgggacc 481 agcacgacct gacctatggg ccgttcacca cggaaggaca ccactggtac cagctgcgcc 541 aggctctgaa ccagcggttg ctgaagccag cggaagcagc gctctatacg gatgctttca 601 atgaggtgat tgatgacttt atgactcgac tggaccagct gcgggcagag agtgcttcgg 661 ggaaccaggt gtcggacatg gctcaactct tctactactt tgccttggaa gctatttgct 721 acatcctgtt cgagaaacgc attggctgcc tgcagcgatc catccccgag gacaccgtga 781 ccttcgtcag atccatcggg ttaatgttcc agaactcact ctatgccacc ttcctcccca 841 agtggactcg ccccgtgctg cctttctgga agcgatacct ggatggttgg aatgccatct 901 tttcctttgg gaagaagctg attgatgaga agctcgaaga tatggaggcc caactgcagg 961 cagcagggcc agatggcatc caggtgtctg gctacctgca cttcttactg gccagtggac 1021 agctcagtcc tcgggaggcc atgggcagcc tgcctgagct gctcatggct ggagtggaca 1081 cgacatccaa cacgctgaca tgggccctgt accacctctc aaaggaccct gagatccagg 1141 aggccttgca cgaggaagtg gtgggtgtgg tgccagccgg gcaagtgccc cagcacaagg 1201 actttgccca catgccgttg ctcaaagctg tgcttaagga gactctgcgt ctctaccctg 1261 tggTCCCCAC AAACTCCCGG ATCATAGAAA AGGAAATTGA AGTTGATGGC TTCCTCTTCC 1321 CCAAGAACAC CCAGTTTGTG TTCTGCCACT ATGTGGTGTC CCGGGACCCC ACTGCCTTCT 1381 CTGAGCCTGA AAGCTTCCAG CCCCACCGCT GGCTGAGAAA CAGCCAGCCT GCTACCCCCA 1441 GGATCCAGCA CCCATTTGGC TCTGTGCCCT TTGGCTATGG GGTCCGGGCC TGCCTGGGCC 1501 GCAGGATTGC AGAGCTGGAG ATGCAGCTAC TCCTCGCAAG GCTGATCCAG AAGTACAAGG 1561 TGGTCCTGGC CCCGGAGACG GGGGAGTTGA AGAGTGTGGC CCGCATTGTC CTGGTTCCCA 1621 ATAAGAAAGT GGGCCTGCAG TTCCTGCAGA GACAGTGCTG CCCAACTTTC TTGTACAAAG 1681 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1741 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1801 ACGACCTAAC CTGGAGCCGC CGGTATATAC GCGTTAAGTC gacaatcaac ctctggatta 1861 caaaatttgt gaaagatt