Construct: ORF TRCN0000467542
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007900.1_s317c1
- Derived from:
- ccsbBroadEn_05858
- DNA Barcode:
- ACTAACTGGAGAACACTGCCTGGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ASL (435)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467542
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 435 | ASL | argininosuccinate lyase | NM_000048.4 | 99.8% | 99.7% | 6C>G;1375C>A |
| 2 | human | 435 | ASL | argininosuccinate lyase | NM_001024943.2 | 99.8% | 99.7% | 6C>G;1375C>A |
| 3 | human | 435 | ASL | argininosuccinate lyase | NM_001024944.2 | 95.5% | 95.4% | 6C>G;917_918ins60;1315C>A |
| 4 | human | 435 | ASL | argininosuccinate lyase | NM_001024946.2 | 94.2% | 94.1% | 6C>G;521_522ins78;1297C>A |
| 5 | mouse | 109900 | Asl | argininosuccinate lyase | NM_133768.5 | 87% | 93.7% | (many diffs) |
| 6 | mouse | 109900 | Asl | argininosuccinate lyase | XM_006504341.3 | 87% | 93.7% | (many diffs) |
| 7 | mouse | 109900 | Asl | argininosuccinate lyase | XM_017320601.1 | 44.2% | 47.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1458
- ORF length:
- 1392
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gtcggagagt gggaagcttt ggggtggccg gtttgtgggt gcagtggacc 121 ccatcatgga gaagttcaac gcgtccattg cctacgaccg gcacctttgg gaggtggatg 181 ttcaaggcag caaagcctac agcaggggcc tggagaaggc agggctcctc accaaggccg 241 agatggacca gatactccat ggcctagaca aggtggctga ggagtgggcc cagggcacct 301 tcaaactgaa ctccaatgat gaggacatcc acacagccaa tgagcgccgc ctgaaggagc 361 tcattggtgc aacggcaggg aagctgcaca cgggacggag ccggaatgac caggtggtca 421 cagacctcag gctgtggatg cggcagacct gctccacgct ctcgggcctc ctctgggagc 481 tcattaggac catggtggat cgggcagagg cggaacgtga tgttctcttc ccggggtaca 541 cccatttgca gagggcccag cccatccgct ggagccactg gattctgagc cacgccgtgg 601 cactgacccg agactctgag cggctgctgg aggtgcggaa gcggatcaat gtcctgcccc 661 tggggagtgg ggccattgca ggcaatcccc tgggtgtgga ccgagagctg ctccgagcag 721 aactcaactt tggggccatc actctcaaca gcatggatgc cactagtgag cgggactttg 781 tggccgagtt cctgttctgg gcttcgctgt gcatgaccca tctcagcagg atggccgagg 841 acctcatcct ctactgcacc aaggaattca gcttcgtgca gctctcagat gcctacagca 901 cgggaagcag cctgatgccc cagaagaaaa accccgacag tttggagctg atccggagca 961 aggctgggcg tgtgtttggg cggtgtgccg ggctcctgat gacccTCAAG GGACTTCCCA 1021 GCACCTACAA CAAAGACTTA CAGGAGGACA AGGAAGCTGT GTTTGAAGTG TCAGACACTA 1081 TGAGTGCCGT GCTCCAGGTG GCCACTGGCG TCATCTCTAC GCTGCAGATT CACCAAGAGA 1141 ACATGGGACA GGCTCTCAGC CCCGACATGC TGGCCACTGA CCTTGCCTAT TACCTGGTCC 1201 GCAAAGGGAT GCCATTCCGC CAGGCCCACG AGGCCTCCGG GAAAGCTGTG TTCATGGCCG 1261 AGACCAAGGG GGTCGCCCTC AACCAGCTGT CACTGCAGGA GCTGCAGACC ATCAGCCCCC 1321 TGTTCTCGGG CGACGTGATC TGCGTGTGGG ACTACGGGCA CAGTGTGGAG CAGTATGGTG 1381 CCCTGGGCGG CACTGCGCGC TCCAGCGTCG ACTGGCAGAT CCGCCAGGTG CGGGCGCTAA 1441 TGCAGGCACA GCAGGCCTAC TCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1501 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1561 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAACTAACT GGAGAACACT 1621 GCCTGGGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt