Construct: ORF TRCN0000467546
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010930.3_s317c1
- Derived from:
- ccsbBroadEn_14576
- DNA Barcode:
- AAAACAAGAACATTCTAGACCTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CDK6 (1021)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467546
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1021 | CDK6 | cyclin dependent kinase 6 | NM_001145306.1 | 100% | 100% | |
| 2 | human | 1021 | CDK6 | cyclin dependent kinase 6 | NM_001259.8 | 100% | 100% | |
| 3 | human | 1021 | CDK6 | cyclin dependent kinase 6 | XM_006715835.2 | 100% | 100% | |
| 4 | mouse | 12571 | Cdk6 | cyclin-dependent kinase 6 | NM_009873.3 | 89.9% | 96.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1044
- ORF length:
- 978
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gaaggacggc ctgtgccgcg ctgaccagca gtacgaatgc gtggcggaga 121 tcggggaggg cgcctatggg aaggtgttca aggcccgcga cttgaagaac ggaggccgtt 181 tcgtggcgtt gaagcgcgtg cgggtgcaga ccggcgagga gggcatgccg ctctccacca 241 tccgcgaggt ggcggtgctg aggcacctgg agaccttcga gcaccccaac gtggtcaggt 301 tgtttgatgt gtgcacagtg tcacgaacag acagagaaac caaactaact ttagtgtttg 361 aacatgtcga tcaagacttg accacttact tggataaagt tccagagcct ggagtgccca 421 ctgaaaccat aaaggatatg atgtttcagc ttctccgagg tctggacttt cttcattcac 481 accgagtagt gcatcgcgat ctaaaaccac agaacattct ggtgaccagc agcggacaaa 541 taaaactcgc tgacttcggc cttgcccgca tctatagttt ccagatggct ctaacctcag 601 tggtcgtcac gctgtggtac agagcacccg aagtcttgct ccagtccagc tacgccaccc 661 ccgtggatct ctggagtgtt ggctgcatat ttgcagaaat gtttcgtaga aagcctcttt 721 tTCGTGGAAG TTCAGATGTT GATCAACTAG GAAAAATCTT GGACGTGATT GGACTCCCAG 781 GAGAAGAAGA CTGGCCTAGA GATGTTGCCC TTCCCAGGCA GGCTTTTCAT TCAAAATCTG 841 CCCAACCAAT TGAGAAGTTT GTAACAGATA TCGATGAACT AGGCAAAGAC CTACTTCTGA 901 AGTGTTTGAC ATTTAACCCA GCCAAAAGAA TATCTGCCTA CAGTGCCCTG TCTCACCCAT 961 ACTTCCAGGA CCTGGAAAGG TGCAAAGAAA ACCTGGATTC CCACCTGCCG CCCAGCCAGA 1021 ACACCTCGGA GCTGAATACA GCCTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1081 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1141 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA AAACAAGAAC 1201 ATTCTAGACC TTGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1261 att