Transcript: Mouse NM_009873.3

Mus musculus cyclin-dependent kinase 6 (Cdk6), mRNA.

Source:
NCBI, updated 2017-06-10
Taxon:
Mus musculus (mouse)
Gene:
Cdk6 (12571)
Length:
11534
CDS:
328..1308

Additional Resources:

NCBI RefSeq record:
NM_009873.3
NBCI Gene record:
Cdk6 (12571)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_009873.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000378483 CTGAAATGCCTGACGTTTAAT pLKO_005 1159 CDS 100% 15.000 21.000 N Cdk6 n/a
2 TRCN0000023152 CTTGACCACTTACTTGGATAA pLKO.1 639 CDS 100% 10.800 15.120 N Cdk6 n/a
3 TRCN0000023149 CCTGACGTTTAATCCAGCTAA pLKO.1 1167 CDS 100% 4.950 6.930 N Cdk6 n/a
4 TRCN0000378274 CCAAGGAAACCACCTAGTTTA pLKO_005 1447 3UTR 100% 13.200 10.560 N Cdk6 n/a
5 TRCN0000023151 GCAAAGACCTACTTCTGAAAT pLKO.1 1145 CDS 100% 13.200 10.560 N Cdk6 n/a
6 TRCN0000023153 GCTCAACCCATCGAGAAGTTT pLKO.1 1102 CDS 100% 5.625 3.938 N Cdk6 n/a
7 TRCN0000023150 CGGTTGCATCTTTGCAGAAAT pLKO.1 942 CDS 100% 13.200 7.920 N Cdk6 n/a
8 TRCN0000023011 GCAGAGATTGTGGGCAGACTA pLKO.1 82 5UTR 100% 4.950 2.475 Y E030030I06Rik n/a
9 TRCN0000166364 CACACACACACACACACACAA pLKO.1 7735 3UTR 100% 4.950 2.475 Y KAAG1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_009873.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00279 pDONR223 100% 89.9% 96.3% None (many diffs) n/a
2 ccsbBroad304_00279 pLX_304 57.2% 89.9% 96.3% V5 (many diffs) n/a
3 TRCN0000481165 GTGTACGCCCGTGATCTCAGACTC pLX_317 45.8% 89.9% 96.3% V5 (many diffs) n/a
4 ccsbBroadEn_14576 pDONR223 0% 89.9% 96.3% None (many diffs) n/a
5 ccsbBroad304_14576 pLX_304 15.7% 89.9% 96.3% V5 (many diffs) n/a
6 TRCN0000467546 AAAACAAGAACATTCTAGACCTTG pLX_317 33.3% 89.9% 96.3% V5 (many diffs) n/a
7 TRCN0000488331 TGACTTACCTACCTTGAGTTTCTC pLX_317 24.5% 89.9% 96.3% V5 (not translated due to prior stop codon) (many diffs) n/a
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