Construct: ORF TRCN0000467557
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016187.1_s317c1
- Derived from:
- ccsbBroadEn_01336
- DNA Barcode:
- CCTCCCGGGATCTAAAGTTCCACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PTGS2 (5743)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467557
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5743 | PTGS2 | prostaglandin-endoperoxide ... | NM_000963.4 | 100% | 100% | |
| 2 | mouse | 19225 | Ptgs2 | prostaglandin-endoperoxide ... | NM_011198.4 | 85% | 86.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1878
- ORF length:
- 1812
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct cgcccgcgcc ctgctgctgt gcgcggtcct ggcgctcagc catacagcaa 121 atccttgctg ttcccaccca tgtcaaaacc gaggtgtatg tatgagtgtg ggatttgacc 181 agtataagtg cgattgtacc cggacaggat tctatggaga aaactgctca acaccggaat 241 ttttgacaag aataaaatta tttctgaaac ccactccaaa cacagtgcac tacatactta 301 cccacttcaa gggattttgg aacgttgtga ataacattcc cttccttcga aatgcaatta 361 tgagttatgt gttgacatcc agatcacatt tgattgacag tccaccaact tacaatgctg 421 actatggcta caaaagctgg gaagccttct ctaacctctc ctattatact agagcccttc 481 ctcctgtgcc tgatgattgc ccgactccct tgggtgtcaa aggtaaaaag cagcttcctg 541 attcaaatga gattgtggaa aaattgcttc taagaagaaa gttcatccct gatccccagg 601 gctcaaacat gatgtttgca ttctttgccc agcacttcac gcatcagttt ttcaagacag 661 atcataagcg agggccagct ttcaccaacg ggctgggcca tggggtggac ttaaatcata 721 tttacggtga aactctggct agacagcgta aactgcgcct tttcaaggat ggaaaaatga 781 aatatcagat aattgatgga gagatgtatc ctcccacagt caaagatact caggcagaga 841 tgatctaccc tcctcaagtc cctgagcatc tacggtttgc tgtggggcag gaggtctttg 901 gtctggtgcc tggtctgatg atgtatgcca caatctggct gcgggaacac aacagagtat 961 gcgatgtgct taaacaggag catcctgaat ggggtgatga gcagttgttc cagacaagca 1021 ggctaatact gataggagag actattaaga ttgtgattga agattatgtg caacacttga 1081 gtggctatca cttcaaactg aaatttgacc cagaactact tttcaacaaa caattccagt 1141 accaaaatcg tattgctgct gaatttaaca ccctctatca ctggcatccc cttctgcctg 1201 acacctttca aattcatgac cagaaataca actatcaaca gtttatctac aacaactcta 1261 tattgctgga acatggaatt acccagtttg ttgaatcatt caccaggcaa attgctggca 1321 gggttgctgg tggtaggaat gttccacccg cagtacagaa agtatcacag gcttccattg 1381 accagagcag gcagatgaaa taccagtctt ttaatgagta ccgcaaacgc tttatgctga 1441 agccctatga atcatttgaa gaacttacag gagaaaagga aatgtctgca gagttggaag 1501 cactctatgg tgacatcgat gctgtggagc tgtatcctgc ccttctggta gaaaagccTC 1561 GGCCAGATGC CATCTTTGGT GAAACCATGG TAGAAGTTGG AGCACCATTC TCCTTGAAAG 1621 GACTTATGGG TAATGTTATA TGTTCTCCTG CCTACTGGAA GCCAAGCACT TTTGGTGGAG 1681 AAGTGGGTTT TCAAATCATC AACACTGCCT CAATTCAGTC TCTCATCTGC AATAACGTGA 1741 AGGGCTGTCC CTTTACTTCA TTCAGTGTTC CAGATCCAGA GCTCATTAAA ACAGTCACCA 1801 TCAATGCAAG TTCTTCCCGC TCCGGACTAG ATGATATCAA TCCCACAGTA CTACTAAAAG 1861 AACGTTCGAC TGAACTGTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1921 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTGAAAG 1981 TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACCTCCCGG GATCTAAAGT 2041 TCCACGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga aagatt