Construct: ORF TRCN0000467560
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011362.1_s317c1
- Derived from:
- ccsbBroadEn_03348
- DNA Barcode:
- TGTTCAGGGGAATGGGGGAGAGGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DYNC2LI1 (51626)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467560
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51626 | DYNC2LI1 | dynein cytoplasmic 2 light ... | NM_001193464.2 | 100% | 100% | |
| 2 | human | 51626 | DYNC2LI1 | dynein cytoplasmic 2 light ... | NM_016008.4 | 99.7% | 99.7% | 507_508insCAG |
| 3 | human | 51626 | DYNC2LI1 | dynein cytoplasmic 2 light ... | NM_001348913.1 | 90.7% | 90.1% | (many diffs) |
| 4 | human | 51626 | DYNC2LI1 | dynein cytoplasmic 2 light ... | NM_001348912.1 | 90.4% | 89.8% | (many diffs) |
| 5 | human | 51626 | DYNC2LI1 | dynein cytoplasmic 2 light ... | NM_015522.3 | 53.6% | 49.5% | (many diffs) |
| 6 | mouse | 213575 | Dync2li1 | dynein cytoplasmic 2 light ... | NM_172256.1 | 83.7% | 87.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1122
- ORF length:
- 1056
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc cagtgaaact ctctgggaaa ttgcaaaagc tgaagtggaa aaaaggggaa 121 ttaatggaag tgaaggtgat ggagctgaaa ttgcagaaaa atttgttttc ttcattggca 181 gtaaaaatgg gggaaagact actattattc taaggtgtct tgacagagat gaaccaccaa 241 aaccaacctt agctttggaa tatacatatg gaagaagagc aaaagggcac aacacaccaa 301 aagatatcgc tcacttttgg gaactcggtg gaggaacctc tttattggac ttaatcagca 361 tacccatcac aggtgacacc ttacggacgt tttctcttgt tctcgttctg gatctttcaa 421 aacctaatga tctctggccc accatggaaa atctcttgca agccacaaaa agccatgtag 481 acaaagtgat aatgaaactg ggaaagacaa atgctaaagc agtttctgaa atgagacaga 541 agatctggaa taatatgccg aaggatcatc ctcaggatca tgaattaatt gacccatttc 601 cggtacctct ggtcataatt ggaagtaaat atgatgtttt tcaggatttt gagtctgaga 661 agagaaaggt aatatgcaag acacttcgat ttgttgcaca ttattatgga gcatcattaa 721 tgtttaccag taaatcagaa gctctattac taaaaatacg tggagttatc aaccagttgg 781 catttggcat tgacaaaagc aaatcaATAT GTGTGGATCA GAATAAACCG CTGTTTATCA 841 CAGCAGGATT GGATTCTTTC GGTCAAATAG GATCTCCTCC TGTTCCTGAA AATGACATTG 901 GAAAGCTTCA TGCCCACTCA CCTATGGAGT TGTGGAAAAA AGTGTATGAA AAGCTCTTTC 961 CACCAAAGAG TATTAACACG CTGAAAGATA TCAAGGACCC TGCGAGAGAT CCTCAGTATG 1021 CTGAAAATGA AGTCGATGAG ATGAGAATTC AGAAGGATCT GGAACTGGAA CAGTACAAAA 1081 GAAGTTCTTC CAAGTCTTGG AAACAAATCG AGCTTGATTC TTGCCCAACT TTCTTGTACA 1141 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1201 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1261 AGGACGATGT TCAGGGGAAT GGGGGAGAGG CACGCGTTAA GTCgacaatc aacctctgga 1321 ttacaaaatt tgtgaaagat t