Construct: ORF TRCN0000467581
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000864.1_s317c1
- Derived from:
- ccsbBroadEn_05863
- DNA Barcode:
- ATCAACAGTCTTACTCATCACACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATF3 (467)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467581
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 467 | ATF3 | activating transcription fa... | NM_001030287.3 | 99.8% | 99.4% | 533C>A |
| 2 | human | 467 | ATF3 | activating transcription fa... | NM_001674.4 | 99.8% | 99.4% | 533C>A |
| 3 | human | 467 | ATF3 | activating transcription fa... | XM_005273146.1 | 99.8% | 99.4% | 533C>A |
| 4 | human | 467 | ATF3 | activating transcription fa... | XM_011509579.1 | 99.8% | 99.4% | 533C>A |
| 5 | human | 467 | ATF3 | activating transcription fa... | NM_001206484.3 | 68.3% | 67.9% | 0_1ins171;362C>A |
| 6 | human | 467 | ATF3 | activating transcription fa... | NM_001206488.3 | 68.3% | 67.9% | 0_1ins171;362C>A |
| 7 | human | 467 | ATF3 | activating transcription fa... | NM_001040619.3 | 68.2% | 65.5% | (many diffs) |
| 8 | human | 467 | ATF3 | activating transcription fa... | NM_001206486.2 | 52.6% | 50% | (many diffs) |
| 9 | human | 467 | ATF3 | activating transcription fa... | XR_001737203.1 | 18.2% | (many diffs) | |
| 10 | mouse | 11910 | Atf3 | activating transcription fa... | NM_007498.3 | 88.7% | 94.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 609
- ORF length:
- 543
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 tgggcatgat gcttcaacac ccaggccagg tctctgcctc ggaagtgagt gcttctgcca 121 tcgtcccctg cctgtcccct cctgggtcac tggtgtttga ggattttgct aacctgacgc 181 cctttGTCAA GGAAGAGCTG AGGTTTGCCA TCCAGAACAA GCACCTCTGC CACCGGATGT 241 CCTCTGCGCT GGAATCAGTC ACTGTCAGCG ACAGACCCCT CGGGGTGTCC ATCACAAAAG 301 CCGAGGTAGC CCCTGAAGAA GATGAAAGGA AAAAGAGGCG ACGAGAAAGA AATAAGATTG 361 CAGCTGCAAA GTGCCGAAAC AAGAAGAAGG AGAAGACGGA GTGCCTGCAG AAAGAGTCGG 421 AGAAGCTGGA AAGTGTGAAT GCTGAACTGA AGGCTCAGAT TGAGGAGCTC AAGAACGAGA 481 AGCAGCATTT GATATACATG CTCAACCTTC ATCGGCCCAC GTGTATTGTC CGGGCTCAGA 541 ATGGGAGGAC TCCAGAAGAT GAGAGAAACC TCTTTATCCA ACAGATAAAA GAAGGAAAAT 601 TGCAGAGCTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 661 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 721 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAATCAAC AGTCTTACTC ATCACACCAC 781 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt