Construct: ORF TRCN0000467595
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006255.1_s317c1
- Derived from:
- ccsbBroadEn_09458
- DNA Barcode:
- TATAAACATTCTCCCCATGACAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ANKRD22 (118932)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467595
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 118932 | ANKRD22 | ankyrin repeat domain 22 | NM_144590.3 | 99.6% | 99.4% | 235T>C;375C>T |
| 2 | mouse | 52024 | Ankrd22 | ankyrin repeat domain 22 | NM_024204.6 | 84.3% | 85.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 639
- ORF length:
- 573
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg aatcctatac tctgagccca tctgccaagc agcctatcag aatgactttg 121 gacaagtgtg gcggtgggtg aaagaagaca gcagctatgc caacgttcaa gatggcttta 181 atggagacac gccccTGATC TGTGCTTGCA GGCGAGGGCA TGTGAGAATC GTTTCCTTCC 241 TTTTAAGAAG AAATGCTAAT GTCAACCTCA AAAACCAGAA AGAGAGAACC TGCTTGCATC 301 ATGCTGTGAA GAAAAAATTT ACCTTCATTG ATTATCTACT AATTATCCTC TTAATGCCTG 361 TTCTGCTTAT TGGGTATTTC CTCATGGTAT CAAAGACAAA GCAGAATGAG GCTCTTGTAC 421 GAATGCTACT TGATGCTGGT GTCGAAGTTA ATGCTACAGA TTGTTATGGC TGTACCGCAT 481 TACATTATGC CTGTGAAATG AAAAACCAGT CTCTTATCCC TCTGCTCTTG GAAGCCCGTG 541 CAGACCCCAC AATAAAGAAT AAGCATGGTG AGAGCTCACT GGATATTGCA CGGAGATTAA 601 AATTTTCCCA GATTGAATTA ATGCTAAGGA AAGCATTGTA CCCAACTTTC TTGTACAAAG 661 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 721 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 781 ACGATATAAA CATTCTCCCC ATGACAAAAC GCGTTAAGTC gacaatcaac ctctggatta 841 caaaatttgt gaaagatt