Transcript: Mouse NM_024204.6

Mus musculus ankyrin repeat domain 22 (Ankrd22), mRNA.

Source:
NCBI, updated 2017-06-03
Taxon:
Mus musculus (mouse)
Gene:
Ankrd22 (52024)
Length:
1754
CDS:
267..842

Additional Resources:

NCBI RefSeq record:
NM_024204.6
NBCI Gene record:
Ankrd22 (52024)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_024204.6, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000081767 CATTATGTGGATGTTCAAGAT pLKO.1 354 CDS 100% 4.950 6.930 N Ankrd22 n/a
2 TRCN0000081764 CCTGTCTTGCTAATTGGATAT pLKO.1 558 CDS 100% 10.800 7.560 N Ankrd22 n/a
3 TRCN0000081763 CCTCATGGTATCAAAGACAAA pLKO.1 581 CDS 100% 4.950 3.465 N Ankrd22 n/a
4 TRCN0000081765 CTGGCGTAGAAGTTAATGCTA pLKO.1 637 CDS 100% 3.000 2.100 N Ankrd22 n/a
5 TRCN0000081766 CCTGAGAATTGTCTCCTTCCT pLKO.1 422 CDS 100% 2.640 1.848 N Ankrd22 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_024204.6, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09458 pDONR223 100% 84.3% 85.8% None (many diffs) n/a
2 ccsbBroad304_09458 pLX_304 0% 84.3% 85.8% V5 (many diffs) n/a
3 TRCN0000467595 TATAAACATTCTCCCCATGACAAA pLX_317 76.5% 84.3% 85.8% V5 (many diffs) n/a
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