Transcript: Human NM_144590.3

Homo sapiens ankyrin repeat domain 22 (ANKRD22), mRNA.

Source:
NCBI, updated 2019-05-02
Taxon:
Homo sapiens (human)
Gene:
ANKRD22 (118932)
Length:
3858
CDS:
238..813

Additional Resources:

NCBI RefSeq record:
NM_144590.3
NBCI Gene record:
ANKRD22 (118932)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_144590.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000430487 GGATATTGCACGGAGATTAAA pLKO_005 753 CDS 100% 15.000 21.000 N ANKRD22 n/a
2 TRCN0000137425 GCATGTGAGAATCGTTTCCTT pLKO.1 390 CDS 100% 3.000 4.200 N ANKRD22 n/a
3 TRCN0000135003 CTTGTACGAATGCTACTTGAT pLKO.1 586 CDS 100% 4.950 3.960 N ANKRD22 n/a
4 TRCN0000426894 GACTGGATTCTATCTTCATTT pLKO_005 854 3UTR 100% 13.200 9.240 N ANKRD22 n/a
5 TRCN0000413220 TGCTAAGGAAAGCATTGTAAT pLKO_005 794 CDS 100% 13.200 9.240 N ANKRD22 n/a
6 TRCN0000137548 CCATGGCCGTAATCCTTCTAA pLKO.1 999 3UTR 100% 5.625 3.938 N ANKRD22 n/a
7 TRCN0000081763 CCTCATGGTATCAAAGACAAA pLKO.1 552 CDS 100% 4.950 3.465 N Ankrd22 n/a
8 TRCN0000138052 CCAGAAAGAGAGAACCTGCTT pLKO.1 447 CDS 100% 2.640 1.848 N ANKRD22 n/a
9 TRCN0000137680 GCCTGTTCTGCTTATTGGGTA pLKO.1 528 CDS 100% 2.640 1.848 N ANKRD22 n/a
10 TRCN0000134806 CTTCAGTCAGAAACTGATGTT pLKO.1 1049 3UTR 100% 0.495 0.347 N ANKRD22 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_144590.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09458 pDONR223 100% 99.6% 99.4% None 235T>C;375C>T n/a
2 ccsbBroad304_09458 pLX_304 0% 99.6% 99.4% V5 235T>C;375C>T n/a
3 TRCN0000467595 TATAAACATTCTCCCCATGACAAA pLX_317 76.5% 99.6% 99.4% V5 235T>C;375C>T n/a
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