Construct: ORF TRCN0000467597
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012214.1_s317c1
- Derived from:
- ccsbBroadEn_12049
- DNA Barcode:
- AACACGGAGAAACTCTCCCACCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PUS7 (54517)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467597
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54517 | PUS7 | pseudouridine synthase 7 | XM_017012368.2 | 57.3% | 57.1% | 1_399del;718_719ins102 |
| 2 | human | 54517 | PUS7 | pseudouridine synthase 7 | NM_001318164.2 | 39.1% | 39.1% | 1_1206del |
| 3 | human | 54517 | PUS7 | pseudouridine synthase 7 | NM_019042.5 | 39.1% | 39.1% | 1_1206del |
| 4 | human | 54517 | PUS7 | pseudouridine synthase 7 | NM_001318163.1 | 38.8% | 38.8% | 1_1224del |
| 5 | human | 54517 | PUS7 | pseudouridine synthase 7 | XM_005250462.4 | 38.8% | 38.8% | 1_1224del |
| 6 | human | 54517 | PUS7 | pseudouridine synthase 7 | XM_017012367.2 | 38.8% | 38.8% | 1_1224del |
| 7 | human | 54517 | PUS7 | pseudouridine synthase 7 | XM_011516336.3 | 33.7% | 33.5% | 1_1224del;1543_1544ins102 |
| 8 | human | 54517 | PUS7 | pseudouridine synthase 7 | XR_927481.3 | 20% | 1_1447del;1566_1567ins73;2152_3433del | |
| 9 | mouse | 78697 | Pus7 | pseudouridylate synthase 7 | NM_001289781.1 | 34.1% | 36.6% | (many diffs) |
| 10 | mouse | 78697 | Pus7 | pseudouridylate synthase 7 | NM_178403.5 | 34.1% | 36.6% | (many diffs) |
| 11 | mouse | 78697 | Pus7 | pseudouridylate synthase 7 | NM_001289780.1 | 33.8% | 36.3% | (many diffs) |
| 12 | mouse | 78697 | Pus7 | pseudouridylate synthase 7 | XR_880846.2 | 18% | (many diffs) | |
| 13 | mouse | 78697 | Pus7 | pseudouridylate synthase 7 | XR_001784757.1 | 13.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 843
- ORF length:
- 777
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tttaatattg aaaccccgct ctggagctga aaagggctac ttggttaaat 121 gcagagaaga atgggcaaag accaaagacc caactgctgc cctcagaaaa ctacctgtca 181 aaaggtgtgt ggaagggcag ctgcttcgag gactttcaaa atatggaatg aagaatatag 241 tctctgcatt tggcataata cccagaaata atcgcttaat gtatattcat agctaccaaa 301 gctatgtgtg gaataacatg gtaagcaaga ggatagaaga ctatggacta aaaccTGTTC 361 CAGGGGACCT CGTTCTCAAA GGAGCCACAG CCACCTATAT TGAGGAAGAT GATGTTAATA 421 ATTACTCTAT CCATGATGTG GTAATGCCCT TGCCTGGTTT CGATGTTATC TACCCAAAGC 481 ATAAAATTCA AGAAGCCTAC AGGGAAATGC TCACAGCTGA CAATCTTGAT ATTGACAACA 541 TGAGACACAA AATTCGAGAT TATTCCTTGT CAGGGGCCTA CCGAAAGATC ATTATTCGTC 601 CTCAGAATGT TAGCTGGGAA GTCGTTGCAT ATGATGATCC CAAAATTCCA CTTTTCAACA 661 CAGATGTGGA CAACCTAGAA GGGAAGACAC CACCAGTTTT TGCTTCTGAA GGCAAATACA 721 GGGCTCTGAA AATGGATTTT TCTCTACCCC CTTCTACTTA CGCCACCATG GCCATTCGAG 781 AAGTGCTAAA AATGGATACC AGTATCAAGA ACCAGACGCA GCTGAATACA ACCTGGCTTC 841 GCTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 901 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 961 GGCTTTATAT ATCTTGTGGA AAGGACGAAA CACGGAGAAA CTCTCCCACC TTACGCGTTA 1021 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt