Construct: ORF TRCN0000467618
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005139.1_s317c1
- Derived from:
- ccsbBroadEn_09312
- DNA Barcode:
- ATGTCAATGGGCTATTTGCCCCGG
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MSANTD3 (91283)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467618
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 91283 | MSANTD3 | Myb/SANT DNA binding domain... | NM_001198805.1 | 99.8% | 99.6% | 819delT |
| 2 | human | 91283 | MSANTD3 | Myb/SANT DNA binding domain... | NM_001198806.1 | 99.8% | 99.6% | 819delT |
| 3 | human | 91283 | MSANTD3 | Myb/SANT DNA binding domain... | NM_080655.2 | 99.8% | 99.6% | 819delT |
| 4 | human | 91283 | MSANTD3 | Myb/SANT DNA binding domain... | NM_001198807.1 | 54.6% | 53% | (many diffs) |
| 5 | mouse | 66665 | Msantd3 | Myb/SANT-like DNA-binding d... | NM_001145924.1 | 86.5% | 96% | (many diffs) |
| 6 | mouse | 66665 | Msantd3 | Myb/SANT-like DNA-binding d... | NM_001145925.1 | 86.5% | 96% | (many diffs) |
| 7 | mouse | 66665 | Msantd3 | Myb/SANT-like DNA-binding d... | NM_028137.3 | 86.5% | 96% | (many diffs) |
| 8 | mouse | 66665 | Msantd3 | Myb/SANT-like DNA-binding d... | XM_006538187.2 | 86.5% | 96% | (many diffs) |
| 9 | mouse | 66665 | Msantd3 | Myb/SANT-like DNA-binding d... | XM_006538188.3 | 86.5% | 96% | (many diffs) |
| 10 | mouse | 66665 | Msantd3 | Myb/SANT-like DNA-binding d... | XM_011250086.2 | 86.5% | 96% | (many diffs) |
| 11 | mouse | 66665 | Msantd3 | Myb/SANT-like DNA-binding d... | XM_011250087.2 | 86.5% | 96% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 891
- ORF length:
- 825
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca aaacaacgaa attataaagc ctgccaaata cttctcagaa ttggaaaaga 121 gcatcctgct ggctttagta gaaaagtata aatatgtgct ggaatgtaag aaaagtgatg 181 cgcgaactat tgcccttaag cagcgtacct ggcaggcgct ggcccacgaa tacaactctc 241 agcccagcgt gtccctgcgg gatttcaaac agctgaagaa gtgctgggag aacatcaagg 301 ctcggaccaa aaaaattatg gcccatgaaa ggagagagaa agtgaaacgg agcgtcagcc 361 ctctcctgag tacccacgtc ctagggaagg agaagatcgc cagcatgctg ccggagcagc 421 tctacttcct gcagagcccc ccggaggagg agcccgaata ccaccccgac gcctcagccc 481 aagaatcatt tgctgtttca aatagagaac tgtgcgatga tgagaaagag ttcatacatt 541 ttccagtatg tgaggggacc tctcaacctg aaccctcgtg ttcagctgtc agaataacag 601 ccaataaaaa ctacaggagc aaaacctctc aggaaggtgc tttaaaaaag atgcatgagg 661 aagaacacca tcaacaaatg tccaTCTTAC AACTGCAACT GATACAAATG AATGAGGTGC 721 ATGTGGCCAA AATCCAGCAG ATAGAGCGAG AGTGTGAGAT GGCAGAGGAG GAACACAGGA 781 TAAAAATGGA AGTTCTCAAT AAAAAGAAGA TGTATTGGGA AAGAAAACTA CAAACTTTTA 841 CCAAGGAATG GCCTGTTTCC TCATTTAACC GGCCCTTTCC CAATCGCCCT ACCCAACTTT 901 CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC 961 TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC 1021 TTGTGGAAAG GACGAATGTC AATGGGCTAT TTGCCCCGGA CGCGTTAAGT Cgacaatcaa 1081 cctctggatt acaaaatttg tgaaagatt