Construct: ORF TRCN0000467624

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018929.2_s317c1
Derived from:
ccsbBroadEn_15227
DNA Barcode:
CACATTAATACTTGACATGAGCAA
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
ALPK2 (115701)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000467624
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 115701 ALPK2 alpha kinase 2 NM_052947.4 88.7% 12.3% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
909
ORF length:
840
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggcatcaaag ttcacggatg gtgacctgaa caatgatggt cctcatgatg
121aaggcttacg ctctagtcag caaaatccca aagtacagaa atacattagc ttcagcctcc
181cgctgtctga ggcaactgca cacatttacc caggtgacag tgccgtggcc aacaaacaac
241ccagcccaca gctttccagt gaagactctg acagtgacta tgaactttgc ccagagataa
301ccctaaccta caccgaggag ttttcagatg atgacctgga gtatctggaa tgttctgatg
361ttatgacgga ttactctaat gcagtttggc aaaggaacct gctggggact gagcatgttt
421ttttattaga aagcgatgac gaagagatgg aattcggtga gcattgcctg ggtgggtgtg
481agcatttcct cagtggaatg ggttgtgggt ctcgggtgtc gggtgacgct gggcctatgg
541ttgccactgc tggcttctgt ggtcatcact cacaacccca agaagttggg gtgaggagca
601gcagagtctc caagcacggt ccctcatccc cacaaacagg gatgactctc attttgggac
661ctcaccagga tggaacgtct tcagtgacag aacaggggag atataaactc cccactgctc
721ccgaggctgc tgaaaatgat tatccaggaa ttcaaggaga aaccagagac agccaccaag
781caagagagga atttgccagt gacaatctgc tcaacatgga tgaatcagta agagagacag
841agatgaagct ccttgtctgg tggagtcaga aaaacttcag ggatggagca gtgttgggag
901acggcagctg acaagagagt ggggggaaag gacttatgga gcaagagggg ttcaaggaaa
961tctgccaggg tgaggcagcc gggaatgaag ggaaatccca agaagccgaa tgccaacctg
1021agagaaagta caacagaagg tacccttcat ctctgctctg ccaaagaatc tgctgagccc
1081ccactaaccc agagtgataa aagagagact tctcacacca cagcagcagc gactggtcgg
1141agttcccatg ctgatgcaag agaatgtgct atttcaaccc aggcagagca agaagcaaaa
1201acccttcaaa cttcaacaga ctcagtctcc aaagaaggca acacaaattg caagggagaa
1261ggcatgcaag ttaatactct atttgaaaca agccaggttc cagactggag tgatcctcct
1321caggtacaag ttcaggaaac agtcagagag acaatctctt gcagccagat gccagctttc
1381tcagagcctg ctggggagga gtccccattc actgggacca caacaatttc cttctcaaac
1441ttaggagggg tccacaagga aaatgcatca ttagctcaac actcggaggt caaaccctgt
1501acctgtggtc cacagcatga agaaaaacaa gacagagatg gcaacatacc tgacaatttc
1561agggaagacc taaaatatga gcagagcatc tcagaagcca atgatgagac tatgtcccca
1621ggtgtgttct caaggcatct ccccaaggat gctcgtgctg acttcaggga gcctgtggct
1681gtctctgttg cttcccctga acccacagat actgccctca ccctggaaaa tgtgtgtgat
1741gagccaaggg acagagaagc agtgtgtgca atggagtgtt ttgaggctgg tgaccaagga
1801acgtgttttg ataccataga ttctcttgtt gggagaccag ttgataaata ttcgcctcaa
1861gaaatttgct ctgtagatac ggaactggca gaaggtcaaa acaaagtatc tgatttatgt
1921tcttctaatg acaagacact ggaagtcttt tttcagacac aagtgtctga gacttcagtg
1981tctacgtgca aaagcagcaa ggacggcaac tcagtcatgt cccctctttt tatcagtact
2041ttcaccttga acatttcaca cacagctagt gaaggtgcca caggagaaaa tctagccaag
2101gtggagaatt ccacctaccc actggcctcc acagtacatg ctggccagga gcagccaagc
2161cccagcaact caggagggct tgatgaaaca cagctccttt cttctgagaa caatccttta
2221gtgcaattta aagaaggagg tgacaagagc cccagtccta gtgccgcaga caccacagcc
2281acaccagcca gttatagttc aattgtgagt tttccttggg agaagccaac aacattaact
2341gctaataatg agtgctttca agcgaccagg gagactgagg acacatcaac tgttaccatt
2401gccaccgaag tccacccagc caaatacctt gctgtgtcaa ttcctgagga caagcatgca
2461ggtggcactg aggagaggtt ccctcgtgca tcccatgaaa aggtttccca atttccttcc
2521caagtgcagg tggatcatat tttaagtggt gctaccatca aatctacaaa agagctactt
2581tgcagggcac ccagtgtgcc aggagtccca caccatgtcc tgcagctccc agagggagag
2641ggtttctgca gtaattcccc tcttcaggtt gataacctgt ctggagataa gagccagact
2701gtggacagag cagactttag gagctatgaa gagaatttcc aagaaagagg aagtgaaaca
2761aagcaggggg tccagcagca gagcctgtcc cagcagggtt ctctttctgc acctgatttc
2821caacaaagtt tgcctacgac atctgctgca caagaggaaa gaaacttggt gcccacggcc
2881ccctcaccag caagctctag ggaaggagca gggcagcgct caggttgggg gacgagggtc
2941tccgtggtgg ctgaaactgc tggggaagaa gacagtcagg ctctgagcaa cgttccatct
3001ctctctgata tccttttgga agagtctaaa gaatatagac ctggaaattg ggaggcaggc
3061aacaagctga agattataac tctagaggct tccgcttctg aaatctggcc accacgacaa
3121ctgacaaatt ctgagagcaa ggcatcagac ggtggtctca taattcctga caaggtctgg
3181gctgtacctg atagtctaaa ggcagatgct gttgtgcctg aattggcccc ctctgaaata
3241gcagcattgg ctcacagtcc agaggatgct gagtcagccc ttgctgatag cagagaaagc
3301cataaaggcg aagagcccac catcagtgta cactggagaa gtctttcttc ccggggtttc
3361agccaaccga gactcctgga gtcatccgtg gaccctgtag atgaaaagga gttatctgtc
3421acagattcac tgtcagcggc ttctgaaact ggagggaagg aaaatgttaa caatgtgagt
3481caagaccagg aggaaaaaca actcaagatg gatcacactg ccttctttaa aaagtttctg
3541acctgcccta aaatcctaga gtcctctgta gatcccattg atgagataag tgtgatagag
3601tacaccaggg ctggaaaacc agagccctct gaaaccacac cacagggcgc cagagaaggg
3661ggtcaatcaa atgacggaaa catgggccac gaagcggaaa tccagccggc cattttgcaa
3721gttccatgtc tccagggaac cattctgagt gaaaatagaa tcagcagaag ccaagaaggc
3781agtatgaagc aggaggcaga acaaattcaa cctgaggagg caaaaactgc catttggcaa
3841gtcctgcaac ccagcgaagg cggtgaaaga attccaagtg gatgtagcat aggccaaata
3901caagaaagca gtgatgggag cttaggggag gctgagcaaa gcaaaaagga caaagcagaa
3961ttgatttccc ccacttcacc tctttctagt tgtcttccaa taatgactca cgcttctctt
4021ggggttgaca cgcacaactc cacaggccaa attcatgacg tccctgaaaa tgacatagtt
4081gagcccagaa agcgtcagta tgtgtttcct gtttcacaga aaaggggaac tattgagaat
4141gagcgtggga aacctttgcc ctcttctcct gatcttacca ggttcccttg tacttcatct
4201cctgaaggaa atgtcacaga ctttttgata agccacaaaa tggaggaacc taaaatagag
4261gtgcttcaaa ttggggaaac caaaccccca agctcatcta gctcctcagc gaagaccttg
4321gcatttattt caggagaacg tgagttagag aaagccccta agttactgca ggatccatgt
4381caaaagggca ccctgggctg tgcgaaaaag tccagggaga gagagaagtc cctggaagcc
4441cgagcaggca aatcgccagg gaccctcaca gcagtgacgg ggtcagagga ggtcaagagg
4501aagccagaag ccccaggcag tggacattta gctgagggag taaagaagaa aattttgtcc
4561agggtggcag cactgaggct gaaactggaa gaaaaggaaa atatcagaaa gaactcagcc
4621tttcttaaaa agatgcccaa actcgaaaca tcattatcac acacagaaga gaaacaagac
4681ccaaaaaagc catcttgcaa aagagaagga agagctccag tattactgaa aaagatccaa
4741gctgagatgt tccctgaaca ctctggaaat gtaaaattaa gctgccaatt tgcagaaatt
4801catgaagatt ctactatctg ctggacaaaa gattcaaagt ccatagccca agtgcagaga
4861agtgcagggg acaactccac tgtttccttt gccatcgtgc aagccagtcc gaaggaccag
4921ggactctatt actgctgcat caagaacagc tacggaaaag tgactgctga atttaacctc
4981acagctgaag ttctcaaaca gctgtcaagt cgccaggata ctaaaggatg tgaagagatt
5041gaattcagcc aactcatctt caaagaagac ttcctccatg acagctactt ttggggccgc
5101ctgcgtggtc agatcgccac ggaggagctg cactnngaga angggttcac cgcaaagctt
5161tcngcagcac agtgatgcac ggcttcatgc ctgtcttcaa acctggccat gcctgtgtgc
5221ttaaggtgca caatgccatt gcctatggga ccagaaataa tgatgagctc atccaaagga
5281actacaaact cgctgcccag gaatgctatg ttcaaaatac tgccaggtat tatgccaaga
5341tctacgctgc tgaagcacag cctctggaag gctttggaga agtacctgag atcattccta
5401tttttcttat ccatcggcct gagaacaata tcccgtatgc tacagtggag gaggagctga
5461ttggagaatt tgtgaagtat tccatcaggg atgggaaaga aataaacttc ttgagaagag
5521aatcagaagc tggtcagaaa tgttgcacct tccagcactg ggtgtaccag aaaacaagtg
5581gctgcctcct ggtgacggac atgcaaggtg taggaatgaa gctaactgac gttggcatag
5641caacgctgGC TAAAGGGTAC AAGGGATTTA AAGGCAACTG TTCCATGACC TTCATTGATC
5701AGTTTAAAGC ACTACACCAG TGTAACAAGT ATTGCAAAAT GCTGGGACTG AAATCCCTTC
5761AAAACAACAA CCAGAAACAG AAGCAGCCGA GCATTGGGAA AAGCAAAGTT CAAACAAACT
5821CTATGACAGT AAAGAAGGCA GGGCCTGAGA CCCCAGGCGA AAAGAAAACC TTGCCAACTT
5881TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT
5941CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
6001CTTGTGGAAA GGACGACACA TTAATACTTG ACATGAGCAA ACGCGTTAAG TCgacaatca
6061acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)