Construct: ORF TRCN0000467641
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012518.1_s317c1
- Derived from:
- ccsbBroadEn_15439
- DNA Barcode:
- ATCCATCCTGTTACCCCTACTTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GYS1 (2997)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467641
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2997 | GYS1 | glycogen synthase 1 | NM_001161587.2 | 100% | 100% | |
| 2 | human | 2997 | GYS1 | glycogen synthase 1 | NM_002103.5 | 91.3% | 91.3% | 299_490del |
| 3 | human | 2997 | GYS1 | glycogen synthase 1 | NR_027763.2 | 56.8% | (many diffs) | |
| 4 | mouse | 14936 | Gys1 | glycogen synthase 1, muscle | NM_030678.3 | 79.6% | 87.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2085
- ORF length:
- 2019
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc tttaaaccgc actttgtcca tgtcctcact gccaggactg gaggactggg 121 aggatgaatt cgacctggag aacgcagtgc tcttcgaagt ggcctgggag gtggctaaca 181 aggtgggtgg catctacacg gtgctgcaga cgaaggcgaa ggtgacaggg gacgaatggg 241 gcgacaacta cttcctggtg gggccgtaca cggagcaggg cgtgaggacc caggtggaac 301 tgctggaggc ccccaccccg gccctgaaga ggacactgga ttccatgaac agcaagggct 361 gcaagttcct ggcacagagt gaggagaagc cacatgtggt tgctcacttc catgagtggt 421 tggcaggcgt tggactctgc ctgtgtcgtg cccggcgact gcctgtagca accatcttca 481 ccacccatgc cacgctgctg gggcgctacc tgtgtgccgg tgccgtggac ttctacaaca 541 acctggagaa cttcaacgtg gacaaggaag caggggagag gcagatctac caccgatact 601 gcatggaaag ggcggcagcc cactgcgctc acgtcttcac tactgtgtcc cagatcaccg 661 ccatcgaggc acagcacttg ctcaagagga aaccagatat tgtgaccccc aatgggctga 721 atgtgaagaa gttttctgcc atgcatgagt tccagaacct ccatgctcag agcaaggctc 781 gaatccagga gtttgtgcgg ggccattttt atgggcatct ggacttcaac ttggacaaga 841 ccttatactt ctttatcgcc ggccgctatg agttctccaa caagggtgct gacgtcttcc 901 tggaggcatt ggctcggctc aactatctgc tcagagtgaa cggcagcgag cagacagtgg 961 ttgccttctt catcatgcca gcgcggacca acaatttcaa cgtggaaacc ctcaaaggcc 1021 aagctgtgcg caaacagctt tgggacacgg ccaacacggt gaaggaaaag ttcgggagga 1081 agctttatga atccttactg gttgggagcc ttcccgacat gaacaagatg ctggataagg 1141 aagacttcac tatgatgaag agagccatct ttgcaacgca gcggcagtct ttcccccctg 1201 tgtgcaccca caatatgctg gatgactcct cagaccccat cctgaccacc atccgccgaa 1261 tcggcctctt caatagcagt gccgacaggg tgaaggtgat tttccacccg gagttcctct 1321 cctccacaag ccccctgctc cctgtggact atgaggagtt tgtccgtggc tgtcaccttg 1381 gagtcttccc ctcctactat gagccttggg gctacacacc ggctgagtgc acggttatgg 1441 gaatccccag tatctccacc aatctctccg gcttcggctg cttcatggag gaacacatcg 1501 cagacccctc agcttacggt atctacattc ttgaccggcg gttccgcagc ctggatgatt 1561 cctgctcgca gctcacctcc ttcctctaca gtttctgtca gcagagccgg cggcagcgta 1621 tcatccagcg gaaccgcacg gagcgcctct ccgaccttct ggactggaaa tacctaggcc 1681 ggtactatat gtctgcgcgc cacatggcgc tgtccaaggc ctttccagag cacttcaccT 1741 ACGAGCCCAA CGAGGCGGAT GCGGCCCAGG GGTACCGCTA CCCACGGCCA GCCTCGGTGC 1801 CACCGTCGCC CTCGCTGTCA CGACACTCCA GCCCGCACCA GAGTGAGGAC GAGGAGGATC 1861 CCCGGAACGG GCCGCTGGAG GAAGACGGCG AGCGCTACGA TGAGGACGAG GAGGCCGCCA 1921 AGGACCGGCG CAACATCCGT GCACCAGAGT GGCCGCGCCG AGCGTCCTGC ACCTCCTCCA 1981 CCAGCGGCAG CAAGCGCAAC TCTGTGGACA CGGCCACCTC CAGCTCACTC AGCACCCCGA 2041 GCGAGCCCCT CAGCCCCACC AGCTCCCTGG GCGAGGAGCG TAACTACCCA ACTTTCTTGT 2101 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 2161 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 2221 GAAAGGACGA ATCCATCCTG TTACCCCTAC TTTAACGCGT TAAGTCgaca atcaacctct 2281 ggattacaaa atttgtgaaa gatt