Construct: ORF TRCN0000467642
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009710.1_s317c1
- Derived from:
- ccsbBroadEn_04394
- DNA Barcode:
- AGCGCCGATTGAAAAGCCATGATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DCTN5 (84516)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467642
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84516 | DCTN5 | dynactin subunit 5 | NM_032486.4 | 100% | 100% | |
| 2 | human | 84516 | DCTN5 | dynactin subunit 5 | NM_001199743.2 | 83.3% | 82.4% | 451_452insGA;455C>T;456_457ins88 |
| 3 | human | 84516 | DCTN5 | dynactin subunit 5 | XR_001752006.2 | 7.6% | 1_98del;645_7181del | |
| 4 | human | 84516 | DCTN5 | dynactin subunit 5 | XR_001752007.2 | 6.9% | 1_112del;559_588del;689_7885del | |
| 5 | human | 84516 | DCTN5 | dynactin subunit 5 | NR_037573.2 | 4.9% | 1_77del;193_293del;725_11055del | |
| 6 | mouse | 59288 | Dctn5 | dynactin 5 | NM_021608.3 | 92.1% | 99.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 612
- ORF length:
- 546
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gttgggcgag ctgctctaca acaagtctga gtacatcgag acggcatctg 121 ggaacaaagt cagtcgccag tcagtgttgt gtggaagcca gaacatcgtt ctcaatggca 181 agaccattgt gatgaatgac tgtattatcc gaggggatct ggcaaatgta agagttggac 241 gtcattgtgt tgtgaaaagt cgtagtgtca taaggccacC ATTCAAGAAG TTCAGCAAAG 301 GTGTTGCATT CTTTCCTTTA CATATTGGAG ACCATGTCTT TATTGAGGAA GATTGTGTGG 361 TCAACGCAGC ACAGATTGGT TCCTATGTTC ATGTTGGGAA GAACTGTGTG ATTGGGCGCC 421 GATGTGTGTT GAAAGACTGC TGCAAAATTC TTGACAACAC AGTATTACCT CCAGAAACTG 481 TGGTTCCACC ATTCACTGTC TTCTCAGGCT GCCCAGGACT CTTCTCAGGG GAGCTCCCGG 541 AGTGCACTCA GGAGCTGATG ATTGACGTCA CCAAGAGCTA CTACCAGAAG TTTTTGCCCC 601 TGACGCAAGT CTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 661 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 721 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAAGC GCCGATTGAA AAGCCATGAT 781 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t