Transcript: Human NM_032486.4

Homo sapiens dynactin subunit 5 (DCTN5), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-08-07
Taxon:
Homo sapiens (human)
Gene:
DCTN5 (84516)
Length:
10954
CDS:
78..626

Additional Resources:

NCBI RefSeq record:
NM_032486.4
NBCI Gene record:
DCTN5 (84516)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_032486.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000116821 CGTTCTCAATGGCAAGACCAT pLKO.1 179 CDS 100% 2.640 3.696 N DCTN5 n/a
2 TRCN0000296539 ACAGTCACTTTGTACCATTAT pLKO_005 862 3UTR 100% 13.200 9.240 N DCTN5 n/a
3 TRCN0000296599 GTGATGAATGACTGTATTATC pLKO_005 201 CDS 100% 13.200 9.240 N DCTN5 n/a
4 TRCN0000310295 AGACGGCATCTGGGAACAAAG pLKO_005 121 CDS 100% 10.800 7.560 N DCTN5 n/a
5 TRCN0000308262 TGTAAGAGTTGGACGTCATTG pLKO_005 239 CDS 100% 10.800 7.560 N DCTN5 n/a
6 TRCN0000116820 CATGTCTTTATTGAGGAAGAT pLKO.1 345 CDS 100% 4.950 3.465 N DCTN5 n/a
7 TRCN0000116819 GTGTTGAAAGACTGCTGCAAA pLKO.1 438 CDS 100% 4.950 3.465 N DCTN5 n/a
8 TRCN0000290244 GTGTTGAAAGACTGCTGCAAA pLKO_005 438 CDS 100% 4.950 3.465 N DCTN5 n/a
9 TRCN0000116818 GCTGATGATTGACGTCACCAA pLKO.1 566 CDS 100% 2.640 1.848 N DCTN5 n/a
10 TRCN0000155229 GATCAAGACCATCCTGGCTAA pLKO.1 6402 3UTR 100% 4.050 2.025 Y INTS7 n/a
11 TRCN0000138772 GCAGGAGAATCGCTTGAACTT pLKO.1 9943 3UTR 100% 4.950 2.475 Y DCAF11 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_032486.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04394 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_04394 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000467642 AGCGCCGATTGAAAAGCCATGATG pLX_317 60.6% 100% 100% V5 n/a
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