Construct: ORF TRCN0000467646
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012609.1_s317c1
- Derived from:
- ccsbBroadEn_02089
- DNA Barcode:
- TACCGAATAATTTATATCCCACTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AIFM1 (9131)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467646
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9131 | AIFM1 | apoptosis inducing factor m... | NM_004208.4 | 100% | 100% | |
| 2 | human | 9131 | AIFM1 | apoptosis inducing factor m... | NM_145812.2 | 95.2% | 95.1% | (many diffs) |
| 3 | human | 9131 | AIFM1 | apoptosis inducing factor m... | NR_132647.1 | 68.1% | (many diffs) | |
| 4 | human | 9131 | AIFM1 | apoptosis inducing factor m... | NM_001130847.3 | 52.6% | 52.5% | 968A>C;970_971delATinsCG;972_973ins867 |
| 5 | human | 9131 | AIFM1 | apoptosis inducing factor m... | NM_001130846.3 | 44.6% | 44.6% | 0_1ins1017 |
| 6 | mouse | 26926 | Aifm1 | apoptosis-inducing factor, ... | NM_012019.3 | 90.5% | 92.1% | (many diffs) |
| 7 | mouse | 26926 | Aifm1 | apoptosis-inducing factor, ... | NM_001290364.1 | 86% | 87.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1908
- ORF length:
- 1839
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gttccggtgt ggaggcctgg cggcgggtgc tttgaagcag aagctggtgc 121 ccttggtgcg gaccgtgtgc gtccgaagcc cgaggcagag gaaccggctc ccaggcaact 181 tgttccagcg atggcatgtt cctctagaac tccagatgac aagacaaatg gctagctctg 241 gtgcatcagg gggcaaaatc gataattctg tgttagtcct tattgtgggc ttatcaacag 301 taggagctgg tgcctatgcc tacaagacta tgaaagagga tgaaaaaaga tacaatgaaa 361 gaatttcagg gttagggctg acaccagaac agaaacagaa aaaggccgcg ttatctgctt 421 cagaaggaga ggaagttcct caagacaagg cgccaagtca tgttcctttc ctgctaattg 481 gtggaggcac agctgctttt gctgcagcca gatccatccg ggctcgggat cctggggcca 541 gggtactgat tgtatctgaa gatcctgagc tgccgtacat gcgacctcct ctttcaaaag 601 aactgtggtt ttcagatgac ccaaatgtca caaagacact gcgattcaaa cagtggaatg 661 gaaaagagag aagcatatat ttccagccac cttctttcta tgtctctgct caggacctgc 721 ctcatattga gaatggtggt gtggctgtcc tcactgggaa gaaggtagta cagctggatg 781 tgagagacaa catggtgaaa cttaatgatg gctctcaaat aacctatgaa aagtgcttga 841 ttgcaacagg aggtactcca agaagtctgt ctgccattga tagggctgga gcagaggtga 901 agagtagaac aacgcttttc agaaagattg gagactttag aagcttggag aagatttcac 961 gggaagtcaa atcaattacg attatcggtg ggggcttcct tggtagcgaa ctggcctgtg 1021 ctcttggcag aaaggctcga gccttgggca cagaagtgat tcaactcttc cccgagaaag 1081 gaaatatggg aaagatcctc cccgaatacc tcagcaactg gaccatggaa aaagtcagac 1141 gagagggggt taaggtgatg cccaatgcta ttgtgcaatc cgttggagtc agcagtggca 1201 agttacttat caagctgaaa gacggcagga aggtagaaac tgaccacata gtggcagctg 1261 tgggcctgga gcccaatgtt gagttggcca agactggtgg cctggaaata gactcagatt 1321 ttggtggctt ccgggtaaat gcagagctac aagcacgctc taacatctgg gtggcaggag 1381 atgctgcatg cttctacgat ataaagttgg gaaggaggcg ggtagagcac catgatcacg 1441 ctgttgtgag tggaagattg gctggagaaa atatgactgg agctgcTAAG CCGTACTGGC 1501 ATCAGTCAAT GTTCTGGAGT GATTTGGGCC CCGATGTTGG CTATGAAGCT ATTGGTCTTG 1561 TGGACAGTAG TTTGCCCACA GTTGGTGTTT TTGCAAAAGC AACTGCACAA GACAACCCCA 1621 AATCTGCCAC AGAGCAGTCA GGAACTGGTA TCCGATCAGA GAGTGAGACA GAGTCCGAGG 1681 CCTCAGAAAT TACTATTCCT CCCAGCACCC CGGCAGTTCC ACAGGCTCCC GTCCAGGGGG 1741 AGGACTACGG CAAAGGTGTC ATCTTCTACC TCAGGGACAA AGTGGTCGTG GGGATTGTGC 1801 TATGGAACAT CTTTAACCGA ATGCCAATAG CAAGGAAGAT CATTAAGGAC GGTGAGCAGC 1861 ATGAAGATCT CAATGAAGTA GCCAAACTAT TCAACATTCA TGAAGACTTG CCAACTTTCT 1921 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1981 CGTAGTAATG AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC 2041 GATACCGAAT AATTTATATC CCACTCACGC GTTAAGTCga caatcaacct ctggattaca 2101 aaatttgtga aagatt