Construct: ORF TRCN0000467648
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016461.1_s317c1
- Derived from:
- ccsbBroadEn_11995
- DNA Barcode:
- AGGTATGGGCTAAATGCAACGAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GTSE1 (51512)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467648
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51512 | GTSE1 | G2 and S-phase expressed 1 | NM_016426.7 | 97.3% | 97.2% | 1_57del;1573T>C |
| 2 | human | 51512 | GTSE1 | G2 and S-phase expressed 1 | XM_005261627.4 | 97.3% | 97.2% | 1_57del;1573T>C |
| 3 | human | 51512 | GTSE1 | G2 and S-phase expressed 1 | XM_017028816.2 | 75% | 75.1% | (many diffs) |
| 4 | human | 51512 | GTSE1 | G2 and S-phase expressed 1 | XM_011530213.3 | 64.2% | 61.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2226
- ORF length:
- 2160
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggtatgga tgaccctaag aaggaagaca ttcttctttt ggccgatgaa aaatttgact 121 tcgatctttc attgtcttct tcgagtgcaa atgaagatga tgaagtcttc ttcggaccct 181 ttggacataa agaaagatgt attgctgcca gcttggaatt aaataatccg gttcccgaac 241 agcctccgtt gcccacatct gagagtccct ttgcctggag ccctctggcc ggggagaagt 301 tcgtggaggt gtacaaagaa gctcacttac tggctttaca cattgagagc agcagccgga 361 accaggcagc ccaagctgcc aagcctgaag accctcggag ccagggcgtg gaaagattca 421 tacaggagtc aaaattaaaa ataaacctct ttgagaaaga aaaggaaatg aagaaaagcc 481 ccacgtctct taaaagggag acatactacc tgtcagacag ccccttgctg gggccccctg 541 tgggtgagcc tcggctcttg gcctcctccc cggccctgcc cagctctggt gcccaggccc 601 gcctcacccg ggcgccgggg cctccgcact ctgctcatgc tttgcccagg gaatcatgca 661 ctgctcatgc tgcaagtcag gcagcgactc agaggaagcc cgggaccaaa ttgctgctgc 721 ctcgagcggc ctctgttaga ggaagaagca tccctggggc tgcggagaag cccaagaaag 781 agattccagc tagtccttcc aggacaaaaa tcccagctga gaaggaatcc caccgggatg 841 ttctccctga caaacctgcc ccgggtgctg tcaatgtgcc ggccgccgga agccacttgg 901 gccagggcaa gcgggcgatc cctgttccaa acaagttggg gctgaagaag accctgttaa 961 aagcacccgg ctctaccagc aatctcgcaa ggaagtcctc ctcggggcct gtttggagcg 1021 gggcatccag tgcgtgcaca tccccagcag tgggcaaagc taaatcaagt gaatttgcaa 1081 gtattcctgc aaatagctcc cggcctctgt caaacatcag caagtcaggc agaatgggac 1141 ccgccatgct gcggccagct ctgcctgcag gccctgtggg ggcatcctcc tggcaggcca 1201 agcgggtcga tgtttctgag ctggcagcgg agcagctcac ggcacccccc tcagcatccc 1261 ccacccaacc ccagactccg gaaggtggcg gccagtggct gaactccagt tgcgcttggt 1321 cagaatcttc tcaattgaat aagactagaa gtatcagacg gcgagattcc tgtctaaatt 1381 ccaagacaaa ggttatgcct actcctacaa atcaatttaa aattcctaag ttttctattg 1441 gtgactcccc ggacagctca acaccaaagc tttcgcgggc acagcggccg cagtcgtgca 1501 cgtcagttgg cagggtcact gtccacagca ccccggttag acgctcatct gggccagcac 1561 cacaaagcct gctgagcgca cggcgtgtgt cagccttgcc cacacccgcc agccggcgct 1621 gctctggcct tccaccgatg acccccaaaa cgatgcccag ggccgtgggc tctcccctgt 1681 gtgtgccagc tcggagacgt tcctctgagc cccgcaagaa ctctgcaatg agaactgaac 1741 caacaaggga gagcaacaga aagacagatt ccaggctggt ggatgtgtcc cctgacaggG 1801 GTTCTCCTCC TTCCCGTGTG CCTCAGGCAC TTAACTTTTC TCCAGAGGAA AGCGATTCTA 1861 CTTTCTCCAA AAGTACTGCC ACAGAAGTAG CTCGGGAGGA AGCCAAGCCG GGTGGAGATG 1921 CAGCCCCTAG TGAGGCTCTT CTTGTAGATA TCAAACTGGA ACCACTCGCG GTCACTCCAG 1981 ATGCTGCAAG CCAGCCCCTC ATTGACCTTC CTCTCATCGA CTTCTGCGAT ACCCCAGAAG 2041 CACACGTGGC TGTAGGATCT GAAAGCAGGC CTCTGATCGA CCTCATGACA AACACTCCAG 2101 ACATGAATAA AAATGTGGCC AAACCTTCAC CGGTGGTGGG ACAGCTCATA GACCTGAGCT 2161 CCCCTCTGAT CCAGCTGAGC CCTGAGGCTG ACAAGGAGAA CGTGGATTCC CCACTCCTCA 2221 AGTTCTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 2281 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 2341 CTTGGCTTTA TATATCTTGT GGAAAGGACG AAGGTATGGG CTAAATGCAA CGAACACGCG 2401 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt