Construct: ORF TRCN0000467656
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005016.1_s317c1
- Derived from:
- ccsbBroadEn_03649
- DNA Barcode:
- GTTGGCCCCAGAGGCCTTATAGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM30A (55754)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467656
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55754 | TMEM30A | transmembrane protein 30A | NM_001143958.2 | 100% | 100% | |
| 2 | human | 55754 | TMEM30A | transmembrane protein 30A | NM_018247.4 | 90% | 90% | 235_342del |
| 3 | mouse | 69981 | Tmem30a | transmembrane protein 30A | NM_133718.4 | 79.1% | 79.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1041
- ORF length:
- 975
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 tcggcatggc gatgaactat aacgcgaagg atgaagtgga cggtgggccc ccgtgtgctc 121 cggggggcac cgcgaagact cggagaccgg ataacacggc cttcaaacag caacggctgc 181 cagcttggca gcccatcctt acggctggca cggtgctacc tattttcttc atcatcggtc 241 tcatcttcat tcccatcggc attggcattt ttgtcacctc caacaacatc cgcgagatcg 301 agggcaacgt gtttatgtat tatggactgt ctaatttcta tcaaaaccat cgtcgttacg 361 tgaaatctcg agatgatagt caactaaatg gagattctag tgctttgctt aatcccagta 421 aggaatgtga accttatcga agaaatgaag acaaaccaat tgctccttgt ggagctattg 481 ccaacagcat gtttaatgat acattagaat tgtttctcat tggcaatgat tcttatccta 541 tacctatcgc tttgaaaaag aaaggtattg cttggtggac agataaaaat gtgaaattca 601 gaaatccccc tggaggagac aacctggaag aacgatttaa aggtacaaca aagcctgtga 661 actggcttaa accagtttac atgctggatt ctgacccaga taataatgga ttcataaatg 721 aggattttat tgtttGGATG CGTACTGCAG CATTACCTAC TTTTCGCAAG TTGTATCGTC 781 TTATAGAAAG GAAAAGTGAT TTACATCCAA CATTACCAGC TGGCCGATAC TCTTTGAATG 841 TCACATACAA TTACCCTGTA CATTATTTTG ATGGACGAAA ACGGATGATC TTGAGCACTA 901 TTTCATGGAT GGGAGGAAAA AATCCATTTT TGGGGATTGC TTACATCGCT GTTGGATCCA 961 TCTCCTTCCT TCTGGGAGTT GTACTGCTAG TAATTAATCA TAAATATAGA AACAGTAGTA 1021 ATACAGCTGA CATTACCATT TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1081 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1141 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGTTG GCCCCAGAGG 1201 CCTTATAGCA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt