Construct: ORF TRCN0000467664
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002563.1_s317c1
- Derived from:
- ccsbBroadEn_04273
- DNA Barcode:
- CTCTTTCTAGTGCCTTTGCTTAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DOHH (83475)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467664
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83475 | DOHH | deoxyhypusine hydroxylase | NM_001145165.2 | 100% | 100% | |
| 2 | human | 83475 | DOHH | deoxyhypusine hydroxylase | NM_031304.5 | 100% | 100% | |
| 3 | human | 83475 | DOHH | deoxyhypusine hydroxylase | XM_011528341.2 | 47% | 40% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 972
- ORF length:
- 906
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 tttgcatggt gacggagcag gaggtggatg ccatcgggca gacgctggtg gaccccaagc 121 agcccctgca ggcccgcttc cgggcgctgt tcacgctgcg tgggctcggc ggcccaggcg 181 ccattgcatg gatcagccag gccttcgatg acgattccgc cctgctcaag cacgagctgg 241 cctactgcct gggccagatg caggatgccc gcgccatccc catgctggtg gacgtgctgc 301 aagacacccg tcaggagccc atggtgcgcc atgaggcagg ggaggccctg ggggccatcg 361 gggacccgga agttctggag atcctgaagc agtattcctc ggaccccgtc atcgaggtgg 421 ccgagacctg ccagctggcc gtgcgcaggc tggagtggct gcagcagcac ggcggggagc 481 cggcggcggg accctacctc tccgtggacc ctgccccgcc ggctgaggag cgtgacgtgg 541 ggcgcctgcg ggaggcgctg ctggatgagt cccggccgcT CTTCGAGCGA TACCGCGCCA 601 TGTTCGCCCT GCGCAACGCG GGAGGCGAGG AGGCCGCCCT GGCGCTGGCC GAGGGTCTGC 661 ACTGTGGGAG CGCCCTCTTC CGCCACGAGG TCGGCTACGT CCTGGGACAG CTGCAGCACG 721 AGGCGGCGGT GCCCCAGCTG GCGGCCGCCC TGGCCCGATG CACCGAGAAC CCCATGGTGC 781 GGCACGAGTG CGCGGAGGCC CTGGGCGCCA TTGCCCGGCC CGCCTGCCTG GCCGCGCTGC 841 AGGCTCACGC GGACGACCCA GAGCGCGTGG TGCGTGAGAG CTGCGAGGTG GCTCTGGACA 901 TGTATGAGCA CGAGACCGGG CGGGCCTTCC AGTACGCGGA CGGCCTGGAG CAGCTGCGCG 961 GGGCCCCCTC CTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1021 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1081 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACTC TTTCTAGTGC CTTTGCTTAA 1141 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t