Construct: ORF TRCN0000467668
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002813.1_s317c1
- Derived from:
- ccsbBroadEn_04044
- DNA Barcode:
- CGAGCACGGACTCTCTCACACTCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MLPH (79083)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467668
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79083 | MLPH | melanophilin | NM_001042467.3 | 100% | 100% | |
| 2 | human | 79083 | MLPH | melanophilin | NM_024101.7 | 95.3% | 95.3% | 1019_1102del |
| 3 | human | 79083 | MLPH | melanophilin | XM_017004893.1 | 94.6% | 93.3% | (many diffs) |
| 4 | human | 79083 | MLPH | melanophilin | XM_006712740.1 | 93% | 93% | 555_556ins120 |
| 5 | human | 79083 | MLPH | melanophilin | XM_006712737.1 | 88.6% | 88.6% | 555_556ins120;899_982del |
| 6 | human | 79083 | MLPH | melanophilin | XM_017004894.2 | 87.8% | 86.3% | (many diffs) |
| 7 | human | 79083 | MLPH | melanophilin | XM_006712739.1 | 86.6% | 86.6% | 1019_1102del;1287_1288ins156 |
| 8 | human | 79083 | MLPH | melanophilin | NM_001281473.2 | 83.9% | 83.9% | 555_556ins120;1083_1084ins156 |
| 9 | human | 79083 | MLPH | melanophilin | NM_001281474.2 | 79.8% | 79.8% | 672_673ins345 |
| 10 | human | 79083 | MLPH | melanophilin | XM_011511812.1 | 71% | 70.5% | (many diffs) |
| 11 | human | 79083 | MLPH | melanophilin | NR_104019.2 | 43.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1782
- ORF length:
- 1716
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gaagaaactg gatctttcca agctcactga tgaagaggcc cagcatgtct 121 tggaagttgt tcaacgagat tttgacctcc gaaggaaaga agaggaacgg ctagaggcgt 181 tgaagggcaa gattaagaag gaaagctcca agagggagct gctttccgac actgcccatc 241 tgaacgagac ccactgcgcc cgctgcctgc agccctacca gctgcttgtg aatagcaaaa 301 ggcagtgcct ggaatgtggc ctcttcacct gcaaaagctg tggccgcgtc cacccggagg 361 agcagggctg gatctgtgac ccctgccatc tggccagagt cgtgaagatc ggctcactgg 421 agtggtacta tgagcatgtg aaagcccgct tcaagaggtt cggaagtgcc aaggtcatcc 481 ggtccctcca cgggcggctg cagggtggag ctgggcctga actgatatct gaagagagaa 541 gtggagacag cgaccagaca gatgaggatg gagaacctgg ctcagaggcc caggcccagg 601 cccagccctt tggcagcaaa aaaaagcgcc tcctctccgt ccacgacttc gacttcgagg 661 gagactcaga tgactccact cagcctcaag gtcactccct gcacctgtcc tcagtccctg 721 aggccaggga cagcccacag tccctcacag atgagtcctg ctcagagaag gcagcccctc 781 acaaggctga gggcctggag gaggctgata ctggggcctc tgggtgccac tcccatccgg 841 aagagcagcc gaccagcatc tcaccttcca gacacggcgc cctggctgag ctctgcccgc 901 ctggaggctc ccacaggatg gccctgggga ctgctgctgc actcgggtcg aatgtcatca 961 ggaatgagca gctgcccctg cagtacttgg ccgatgtgga cacctctgat gaggaaagca 1021 tccgggctca cgtgatggcc tcccaccatt ccaagcggag aggccgggcg tcttctgaga 1081 gtcagggtct aggtgctgga gtgcgcacgg aggccgatgt agaggaggag gccctgagga 1141 ggaagctgga ggagctgacc agcaacgtca gtgaccagga gacctcgtcc gaggaggagg 1201 aagccaagga cgaaaaggca gagcccaaca gggacaaatc agttgggcct ctcccccagg 1261 cggacccgga ggtgggcacg gctgcccatc aaaccaacag acaggaaaaa agcccccagg 1321 accctgggga ccccgtccag tacaacagga ccacagatga ggagctgtca gagctggagg 1381 acagagtggc agtgacggcc TCAGAAGTCC AGCAGGCAGA GAGCGAGGTT TCAGACATTG 1441 AATCCAGGAT TGCAGCCCTG AGGGCCGCAG GGCTCACGGT GAAGCCCTCG GGAAAGCCCC 1501 GGAGGAAGTC AAACCTCCCG ATATTTCTCC CTCGAGTGGC TGGGAAACTT GGCAAGAGAC 1561 CAGAGGACCC AAATGCAGAC CCTTCAAGTG AGGCCAAGGC AATGGCTGTG CCCTATCTTC 1621 TGAGAAGAAA GTTCAGTAAT TCCCTGAAAA GTCAAGGTAA AGATGATGAT TCTTTTGATC 1681 GGAAATCAGT GTACCGAGGC TCGCTGACAC AGAGAAACCC CAACGCGAGG AAAGGAATGG 1741 CCAGCCACAC CTTCGCGAAA CCTGTGGTGG CCCACCAGTC CTACCCAACT TTCTTGTACA 1801 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1861 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1921 AGGACGACGA GCACGGACTC TCTCACACTC GACGCGTTAA GTCgacaatc aacctctgga 1981 ttacaaaatt tgtgaaagat t