Construct: ORF TRCN0000467677
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010925.1_s317c1
- Derived from:
- ccsbBroadEn_00439
- DNA Barcode:
- GTTGTCACGTCCACTGCCCATTAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TIMM8A (1678)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467677
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1678 | TIMM8A | translocase of inner mitoch... | NM_004085.4 | 100% | 100% | |
| 2 | human | 1678 | TIMM8A | translocase of inner mitoch... | NM_001145951.2 | 48.2% | 45.3% | 134_135delTTinsAGAAGTGCATGGACAAG;140delT;144_145ins133 |
| 3 | mouse | 30058 | Timm8a1 | translocase of inner mitoch... | NM_013898.3 | 90.4% | 94.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 381
- ORF length:
- 291
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGATTCCTC CTCCTCTTCC TCCGCGGCGG 121 GTTTGGGTGC AGTGGACCCG CAGTTGCAGC ATTTCATCGA GGTAGAGACT CAAAAGCAGC 181 GCTTCCAGCA GCTGGTGCAC CAGATGACTG AACTTTGTTG GGAGAAGTGC ATGGACAAGC 241 CTGGGCCAAA GTTGGACAGT CGGGCTGAGG CCTGTTTTGT GAACTGCGTT GAGCGCTTCA 301 TTGATACAAG CCAGTTCATC TTGAATCGAC TGGAACAGAC CCAGAAATCC AAGCCAGTTT 361 TCTCAGAAAG CCTTTCTGAC TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 421 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 481 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGTTG TCACGTCCAC 541 TGCCCATTAA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt