Construct: ORF TRCN0000467687
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008823.1_s317c1
- Derived from:
- ccsbBroadEn_09682
- DNA Barcode:
- AATCACCCGCAGAAGCTTTCGACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CMTM8 (152189)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467687
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 152189 | CMTM8 | CKLF like MARVEL transmembr... | NM_178868.5 | 99.4% | 98.8% | 122C>A;204C>A;236T>C |
| 2 | human | 152189 | CMTM8 | CKLF like MARVEL transmembr... | XM_011533416.3 | 87.7% | 87.2% | (many diffs) |
| 3 | human | 152189 | CMTM8 | CKLF like MARVEL transmembr... | XM_017005779.1 | 72.3% | 71.2% | (many diffs) |
| 4 | human | 152189 | CMTM8 | CKLF like MARVEL transmembr... | NM_001320308.1 | 66.2% | 65.8% | 122C>A;147_148ins174 |
| 5 | human | 152189 | CMTM8 | CKLF like MARVEL transmembr... | XM_017005780.1 | 57.6% | 57.2% | 0_1ins219;17T>C |
| 6 | mouse | 70031 | Cmtm8 | CKLF-like MARVEL transmembr... | NM_027294.2 | 86.3% | 91.3% | (many diffs) |
| 7 | mouse | 70031 | Cmtm8 | CKLF-like MARVEL transmembr... | XM_011242999.2 | 50.6% | 52.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 585
- ORF length:
- 519
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggagccgcag cgcgcccgct cgcacacagt caccaccacc gccagctcct 121 tcgcagagaa cttctccacc agcagcagca gcttcgccTA CGACCGGGAG TTCCTCCGCA 181 CCCTGCACGG CTTCCTCATC GTGGCCGAGA TCGTTCTGGG GCTGCTGGTA TGGACGCTTA 241 TTGCTGGAAC TGAGTACTTC CGGGTCCCAG CATTTGGCTG GGTCATGTTT GTAGCTGTAT 301 CTTACTGGGT CCTCACCGTC TTCTTCCTCA TTATCTACAT AACAATGACC TACACCAGGA 361 TTCCCCAGGT GCCCTGGACA ACAGTGGGCC TGTGCTTTAA CGGCAGTGCC TTCGTCTTGT 421 ACCTCTCTGC CGCTGTTGTA GATGCATCTT CCGTCTCCCC TGAGAGGGAC AGTCACAACT 481 TCAACAGCTG GGCGGCCTCA TCGTTCTTTG CCTTCCTGGT CACCATCTGC TACGCTGGAA 541 ATACATATTT CAGTTTTATA GCATGGAGAT CCAGGACCAT ACAGTGCCCA ACTTTCTTGT 601 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 661 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 721 GAAAGGACGA AATCACCCGC AGAAGCTTTC GACGACGCGT TAAGTCgaca atcaacctct 781 ggattacaaa atttgtgaaa gatt