Construct: ORF TRCN0000467693
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013762.1_s317c1
- Derived from:
- ccsbBroadEn_11835
- DNA Barcode:
- GAAACCTAACCACCAAGTTGTTGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NUFIP1 (26747)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467693
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26747 | NUFIP1 | nuclear FMR1 interacting pr... | NM_012345.3 | 55.6% | 55.7% | 1_657del;1434C>T |
| 2 | human | 26747 | NUFIP1 | nuclear FMR1 interacting pr... | XR_941559.2 | 26.9% | 1_704del;1069_1198del;1282_1283ins380 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 894
- ORF length:
- 828
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca tgctcctggc atgaagaaga tcaagttaga cactccagag gaaattgcac 121 ggtggaggga agaaagaagg aaaaactatc caactctggc caatattgaa aggaagaaga 181 agttaaaact tgaaaaggag aagagaggag cagtattgac aacaacacaa tatggcaaga 241 tgaaggggat gtccagacat tcacaaatgg caaagatcag aagtcctggc aagaatcaca 301 aatggaaaaa cgacaattct agacagagag cagtcactgg atcaggcagt cacttgtgtg 361 atttgaagct agaaggtcca ccggaggcaa atgcagatcc tcttggtgtt ttgataaaca 421 gtgattctga gtctgataag gaggagaaac cacaacattc tgtgataccc aaggaagtga 481 caccagccct atgctcacta atgagtagct atggcagtct ttcagggtca gagagtgagc 541 cagaagaaac tcccatcaag actgaagcag acgttttggc agaaaaccag gttcttgata 601 gcagtgctcc taagagtcca agtcaagatg ttaaagcaac tgttagaaat ttttcagaag 661 ccaagagtga gaaccgaaag aaaagctttg aaaaaacaaa ccctaagagg aaaaaagatt 721 atcacaacta tcaaacgtta ttcgaaccaa gaacacacca tccatatctc ttGGAAATGC 781 TTCTAGCTCC GGACATTCGA CATGAAAGAA ATGTGATTTT GCAGTGTGTT CGGTACATCA 841 TTAAAAAAGA CTTTTTTGGA CTGGATACTA ATTCTGCGAA AAGTAAAGAT GTATACCCAA 901 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 961 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1021 TATCTTGTGG AAAGGACGAG AAACCTAACC ACCAAGTTGT TGAACGCGTT AAGTCgacaa 1081 tcaacctctg gattacaaaa tttgtgaaag att